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GE29-0005-93

His Spintrap TALON®

GE Healthcare, 29-0005-93

  •  NACRES NA.56

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Properties

Related Categories GE Healthcare Life Sciences Protein Sample Preparation Formats, Molecular Biology, Native Protein Sample Preparation, Proteomics, SpinTrap Microcentrifuge More...
shelf life   Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
mfr. no.   GE Healthcare, 29-0005-93
packaging   pkg of 50 x 100 μL
bed volume   100 μL
matrix   6% cross-linked agarose
particle size   60-160 μm
cleaning   2 - 14 (Co2+-stripped medium)
working range   3 - 12 (Co2+-stripped medium)
capacity   ≤1 mg binding capacity(histidine-tagged)
  ≥20 mg binding capacity (histidine-tagged protein)(The binding capacity for individual proteins may vary)
storage temp.   2-8°C

Description

General description

His SpinTrap Talon® is a single-use column for rapid screening with small samples of histidine-tagged proteins.

Application

His SpinTrap Talon® is designed for high purity, small-scale purification and screening of histidine-tagged Proteins from clarified cell lysates. His SpinTrap Talon® columns are used together with a standard microcentrifuge and purification takes approximately 10 minutes. The columns make parallel preparations of few or many small-scale cell lysates quick and easy. Prepacked columns enable highly reproducible column to column purification and yield, with RSD < 6%. Leakage of cobalt ions from Talon® Superflow medium is negligible.

Features and Benefits

• Suitable for purification of multiple samples in parallel, such as screening.
• Convenient, ready-to-use single-use spin column, prepacked with 100 μL Talon® Superflow cobalt-IMAC (immobilized metal affinity chromatography) medium offering a different selectivity compared to nickel-charged media.
• With a microcentrifuge, one run takes about 10 minutes.

Analysis Note

www.gelifesciences.com.

Legal Information

SpinTrap is a trademark of Cytiva

Talon is a registered trademark of Clontech

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis (COA)

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Protocols & Articles

Protocols

Characteristics of Ni Sepharose, Ni Sepharose excel, TALON Superflow, and Uncharged IMAC Sepharose Products

Appendix 1, Extracted from Affinity Chromatography Vol. 2: Tagged Proteins, GE Healthcare, 2016  
Keywords: Affinity chromatography, Buffers, Cell culture, Centrifugation, Chromatography, Detergents, Immobilization, Precipitation, Purification, Tagged proteins

Purification of Histidine-Tagged Proteins using TALON Superflow

TALON® Superflow consists of highly cross-linked agarose beads with an immobilized chelating group precharged with Co2+ ions.
Keywords: Affinity chromatography, Buffers, Cell disruption, Chromatography, Filtration, Gene expression, Homogenization, Immobilization, Sample preparations, Sonication, Tagged proteins

Rapid Screening of Histidine-Tagged Proteins using His SpinTrap™ TALON®

His SpinTrap™ TALON® columns are excellent for screening of expression levels and purification conditions prior to scale up. The columns are designed for use in a microcentrifuge. Figure 3.40 shows th...
Keywords: Affinity chromatography, Cell disruption, Centrifugation, Chromatography, Gene expression, Tagged proteins

Troubleshooting Guide for Affinity Chromatography of Tagged Proteins

The troubleshooting guide below addresses problems common to the majority of purification products discussed in this chapter, as well as problems specific to a particular method. In the latter case, th...
Keywords: Addition reactions, Affinity chromatography, Buffers, Cell culture, Cell disruption, Centrifugation, Chromatography, Detergents, Fermentation, PAGE, Precipitation, Protein biosynthesis, Size-exclusion chromatography, Sonication, Tagged proteins, Western blot

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