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GE29-0005-96

His MultiTrap TALON®

GE Healthcare, 29-0005-96

  •  NACRES NA.56

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Properties

Related Categories GE Healthcare Life Sciences Protein Sample Preparation Formats, Molecular Biology, MultiTrap Centrifuge/vacuum, Native Protein Sample Preparation, Proteomics More...
material   polypropylene and polyethylene
shelf life   Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
mfr. no.   GE Healthcare, 29-0005-96
packaging   pkg of 4 x 96-well
size   127.8 mm × 85.5 mm × 30.6 mm
matrix   6% cross-linked agarose
particle size   60-160 μm
cleaning   2 - 14 (Co2+-stripped medium)
working range   3 - 12 (Co2+-stripped medium)
capacity   ≥20 mg binding capacity (histidine-tagged protein)(The binding capacity for individual proteins may vary)
storage temp.   2-8°C

Description

General description

His MultiTrap Talon® is a ready-to-use 96-well filter plate for high reproducibility in expression screening and small-scale parallel purification of histidine-tagged proteins.

Application

His MultiTrap Talon® multiwell plates are designed for high-throughput screening of histidine-tagged Proteins from clarified cell lysates. His MultiTrap Talon® prepacked plates are manufactured with high reproducibility with regards to the well and media volumes in order to enable consistent results. The average purity of His MultiTrap Talon® is > 85% with yields up to 1 mg/well. Leakage of cobalt ions from Talon® Superflow medium is negligible. His MultiTrap Talon® is used manually with vacuum or centrifugation, or in a liquid handling station.

Features and Benefits

• Up to 1 mg of pure histidine-tagged Protein per well.
• Prefilled with 50 μL Talon® Superflow cobalt-IMAC (immobilized metal affinity chromatography) medium for reproducible and high throughput parallel screening, offering a different selectivity compared to nickel-charged media.
• Large sample volumes can be applied all at once.

Analysis Note

www.gelifesciences.com.

Legal Information

MultiTrap is a trademark of Cytiva

Talon is a registered trademark of Clontech

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis (COA)

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Protocols & Articles

Protocols

Characteristics of Ni Sepharose, Ni Sepharose excel, TALON Superflow, and Uncharged IMAC Sepharose Products

Appendix 1, Extracted from Affinity Chromatography Vol. 2: Tagged Proteins, GE Healthcare, 2016  
Keywords: Affinity chromatography, Buffers, Cell culture, Centrifugation, Chromatography, Detergents, Immobilization, Precipitation, Purification, Tagged proteins

Manual and Automated Purification

Chapter 2, Extracted from Affinity Chromatography Vol. 2: Tagged Proteins, GE Healthcare, 2016
Keywords: Affinity chromatography, Centrifugation, Chromatography, Gene expression, Tagged proteins

Purification of Histidine-Tagged Proteins using His MultiTrap TALON

His MultiTrap™ TALON® 96-well filter plates are prepacked with 50 µl of TALON® Superflow per well. Each plate provides highly reproducible high-throughput screening and rapid small-scale purification of...
Keywords: Affinity chromatography, Cell disruption, Centrifugation, Chromatography, Gene expression, Tagged proteins

Purification of Histidine-Tagged Proteins using TALON Superflow

TALON® Superflow consists of highly cross-linked agarose beads with an immobilized chelating group precharged with Co2+ ions.
Keywords: Affinity chromatography, Buffers, Cell disruption, Chromatography, Filtration, Gene expression, Homogenization, Immobilization, Sample preparations, Sonication, Tagged proteins

Troubleshooting Guide for Affinity Chromatography of Tagged Proteins

The troubleshooting guide below addresses problems common to the majority of purification products discussed in this chapter, as well as problems specific to a particular method. In the latter case, th...
Keywords: Addition reactions, Affinity chromatography, Buffers, Cell culture, Cell disruption, Centrifugation, Chromatography, Detergents, Fermentation, PAGE, Precipitation, Protein biosynthesis, Size-exclusion chromatography, Sonication, Tagged proteins, Western blot

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