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GE29-0491-04

HiTrap® MabSelect SuRe

GE Healthcare, 29-0491-04, pack of 1 mL

  •  NACRES NA.56

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Properties

Related Categories Analytical/Chromatography, Antibodies, Antibody Purification and Characterization, HiTrap & PD-10 Cartridges, MabSelect mAb Purification,
shelf life   Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
packaging   pack of 1 mL
mfr. no.   GE Healthcare, 29-0491-04
availability   not available in North America
parameter   <4 mL/min flow rate (The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.)
  42 psi
bed size   7 mm × 25 mm
bed volume   1 mL
column I.D.   7 mm
matrix   highly cross-linked agarose
avg. part. size   85 μm (d₅₀v is the median particle size of the cumulative volume distribution)
cleaning   2 - 13.7 (pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH.)
working range   3 - 12 (pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH.)
capacity   ≥30 mg binding capacity (humanlgG/ml at 2.4 min residence time)(Determined at 10% breakthrough by frontal analysis at a mobile phase velocity of 500 cm/h in a column with a bed height of 20 cm.)
suitability   suitable for bioprocess medium
storage temp.   2-8°C

Description

General description

HiTrap MabSelect SuRe columns are prepacked with MabSelect SuRe, a BioProcess medium for purification of monoclonal antibodies from large sample volumes. The novel alkali-tolerant recombinant Protein A ligand allows cleaning-in-place (CIP).

Features and Benefits

• Withstands rigorous CIP and sanitization procedures with 0.1 to 0.5 M NaOH.
• Novel ligand design gives enhanced protease resistance resulting in lower ligand leakage.
• Oriented coupling of recombinant Protein A to the matrix via an engineered C-terminal cysteine enhances IgG binding capacity.
• Fewer regulatory concerns due to the total absence of mammalian culture in the ligand production and purification.
• Prepacked HiTrap columns for process development, screening of purification conditions, and small-scale purification of Mabs.

Legal Information

Free MabSelect SuRe Ligand
"MabSelect SuRe Ligand Restricted License" and "Cys-rProtein A Ligand Restricted License" are protected by the following patents and equivalent patents and patent applications in other countries: US 6,399,750 and EP 1123389. A free, non-transferable limited license to use this product for internal analytical purposes only accompanies the purchase of the product from a GE Healthcare company and its licensed distributors. Any other use will require a separate license from a GE Healthcare company.

HiTrap is a registered trademark of Cytiva

MabSelect is a trademark of Cytiva

SuRe is a trademark of Cytiva

Safety & Documentation

Safety Information

RIDADR 
NA 1993 / PGIII

Documents

Certificate of Analysis (COA)

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Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Desalting and Buffer Exchange for Affinity Chromatography of Antibodies

Desalting at laboratory scale is a well-proven, simple, and fast method that will rapidly remove low molecular weight contaminants at the same time as transferring the sample into the desired buffer ...
Keywords: Absorption, Affinity chromatography, Buffers, Centrifugation, Chromatography, Detergents, Dialysis, Electrophoresis, Fractionation, Gel electrophoresis, High performance liquid chromatography, Liquid chromatography mass spectrometry, Mass spectrometry, PAGE, Precipitation, Sample preparations, Separation, Size-exclusion chromatography, Titrations

Performing a Purification of IgG Antibodies with MabSelect Sure

This section describes purification of monoclonal antibodies with HiTrap® MabSelect SuRe. The protocol includes a CIP procedure to minimize cross-contamination from different antibodies between purif...
Keywords: Affinity chromatography, Buffers, Chromatography, PAGE, Purification, Sample preparations

Selection Guide for Polymeric Resins in Chromatography & Purification

Sigma-Aldrich carries a vast array of resins, from leading manufacturers; such as, Dow Chemical, Rohm & Haas, Mitsubishi, and Lanxess. Our product line includes adsorbent, ion-exchange (anion / catio...
Keywords: Adsorption, Antibiotics, Combinatorial synthesis, Detergents, Fermentation, Filtration, Hormones, Ion Exchange, Ion exchange resins, Pharmaceutical, Purification, Separation

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