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H0262 Sigma-Aldrich

HAT Media Supplement (50×) Hybri-Max

γ-irradiated, lyophilized powder, BioXtra, suitable for hybridoma

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Properties

Related Categories Cell Culture, Conditioned media supplements and reagents, Hybridoma Platform, Hybridoma Reagents, Reagents and Supplements,
Quality Level   200
sterility   γ-irradiated
product line   BioXtra
form   lyophilized powder
application(s)   cell culture | hybridoma: suitable
impurities   endotoxin, tested
shipped in   dry ice
storage temp.   −20°C

Description

Application

HAT Media Supplement (50×) Hybri-Max has been used:
• in the selection and validation of hypoxanthine-guanine phosophoribosyltransferase (HPRT)-deficient clones
• in the generation of mAbs (monoclonal antibodies)
• in the luciferase-based bioassay (LUC) assay

Reconstitution

Reconstitute contents of vial with 10 ml sterile cell culture medium. Stock solution is sufficient to prepare 500 ml medium. Final working concentration: 100 μM hypoxanthine, 0.4 μM aminopterin, 16 μM thymidine.

Legal Information

Hybri-Max is a trademark of Sigma-Aldrich Co. LLC

Safety & Documentation

Safety Information

Symbol 
Signal word 
Danger
Hazard statements 
Precautionary statements 
Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Protocols & Articles

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Cell Culture Troubleshooting Guide

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Peer-Reviewed Papers
15

References

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92210 Timestrip Plus 0 °C
06693 Timestrip Plus -20 °C

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