H0887 Sigma-Aldrich

HEPES solution

1 M, pH 7.0-7.6, sterile-filtered, BioReagent, suitable for cell culture

Synonym: N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid)



Related Categories Biochemicals and Reagents, Biological Buffers, Buffer Convenience Packaging, Buffer Solutions, Buffers A to Z,
Quality Level   300
sterility   sterile-filtered
product line   BioReagent
form   liquid
concentration   1 M
application(s)   cell culture | mammalian: suitable
impurities   endotoxin, tested
pH   7.0-7.6
useful pH range   6.8 - 8.2
pH range   7.0 - 7.6
storage temp.   2-8°C
InChI   1S/C8H18N2O4S/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14/h11H,1-8H2,(H,12,13,14)


General description

HEPES has been described as one of the best all-purpose buffers available for biological research. At biological pH, the molecule is zwitterionic, and is effective as a buffer at pH 6.8 to 8.2 (pKa 7.55). It is typically used in cell culture at concentration between 5mM to 30 mM. HEPES has been used in a wide variety of applications, including tissue culture. It is commonly used to buffer cell culture media in air. HEPES finds its usage in in vitro experiments on Mg.


HEPES has been used as a component of:
• Hank’s balanced salt solution, Dulbecco’s modified eagle’s medium and no-glucose DMEM, which are used for the preparation of tissue slices
• Buffers used in localized conductance measurements on the basolateral side of culture inserts to examine cation selectivity


Concentrations of HEPES over 40mM may be toxic to some cell types.

Preparation Note

Prepared from HEPES free acid and cell culture grade water.

Safety & Documentation

Safety Information

NONH for all modes of transport
WGK Germany 
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable
Protocols & Articles


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Peer-Reviewed Papers


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