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H1009 Sigma

Hydrogen peroxide solution

30 % (w/w) in H2O, contains stabilizer

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Description

Frequently Asked Questions

Frequently Asked Questions are available for this Product.

Packaging

5, 100, 500 mL in poly bottle

Application

Used to prepare solutions for induction of apoptosis. ~60% of HL60 cells were apoptotic after exposure to 15 μM H2O2 for 8 hr.

Features and Benefits

This compound is featured on the Phosphoprotein Phosphatases (Tyrosine) page of the Handbook of Receptor Classification and Signal Transduction. To browse other handbook pages, click here.

Price and Availability


Added Consistency

Bulk Chemicals
Safety & Documentation

Safety Information

Symbol 
Signal word 
Danger
Hazard statements 
RIDADR 
UN 2014 8(5.1) / PGII
WGK Germany 
1

Documents

Certificate of Analysis

Certificate of Origin

Frequently Asked Questions

What is the molarity of the 30% hydrogen peroxide solution?
The molarity of a 30 % hydrogen peroxide solution is 9.8 M based on a density of 1.11 g/ml.  The answer can also be found by clicking:  http://www.sigmaaldrich.com/sigma-aldrich/product_information_sheet/h1009pis.pdf
How stable is the 30 % hydrogen peroxide solution?
Although dilute solutions of hydrogen peroxide have a limited shelf life, the 30% solution (when properly stored) has a shelf life measured in years. Please see the certificate of analysis for the recommend retest date for a particular lot of Product No. H1009.
Does the hydrogen peroxide contain a stabilizer?
This product contains 0.5 ppm stannate-containing compounds and 1 ppm phosphorus-containing compounds to stabilize the solution.  The answer can also be found by clicking:  http://www.sigmaaldrich.com/etc/medialib/docs/Sigma/productinformationsheet2/h1009pis.Par.0001.File.tmp/h1009pis.pdf
Can hydrogen peroxide be detected spectrophotometrically?
Hydrogen peroxide has a molar extinction coefficient of 43.6 at 240 nm.  The answer can also be found by clicking:  http://www.sigmaaldrich.com/sigma-aldrich/product_information_sheet/h1009pis.pdf  
What is the pH of a 30% hydrogen peroxide solution?
The pH of a 30 % hydrogen peroxide solution is typically between 3 and 4.  The answer can also be found by clicking:  http://www.sigmaaldrich.com/sigma-aldrich/product_information_sheet/h1009pis.pdf
Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis. These documents are located on the product detail page under Useful Links & Tools. Click on the following link to search for a Certificate of Analysis. Please click the following link to see the details on our Product Dating Information.
How do I get lot-specific information or a Certificate of Analysis?
A Certificate of Analysis is available by lot number and can be obtained through our Advanced Search Option: http://www.sigmaaldrich.com/catalog/AdvancedSearchPage.do
How do I find price and availability?
There are several ways to find pricing and availability for our products.  Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers. 
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS. To access the shipping information for this material, use the link on the product detail page for the product, or search here. 
My question is not addressed here, how can I contact Technical Service for assistance?
Use the option to the right to "Ask a Question" by email of a Technical Service Scientist.
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Protocols & Articles

Articles

Discover Bioactive Small Molecules for Nitric Oxide & Cell Stress Research

Exposure to harsh conditions, cellular damage, and other factors can cause stress on cells. Stress attributed directly and indirectly to oxygen is referred to as oxidative stress, which is imposed on...
Keywords: Antimicrobials, Bioactive small molecules, Cancer, Diseases, Neurodegenerative Diseases, Oxidations

HPLC Analysis of Native and Oxidized Filgrastim on BIOshell™ A400 Protein C4 Column

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Native and Oxidized Filgrastim on BIOshell™ A400 Protein C4 Column
Keywords: Chromatography, Genetic, High performance liquid chromatography, Oxidations

HPLC Analysis of an Oxidized Monoclonal Antibody Standard on BIOshell™ A400 Protein C4

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of an Oxidized Monoclonal Antibody Standard on BIOshell™ A400 Protein C4
Keywords: Chromatography, High performance liquid chromatography

Protocols

4-Chloro-1-Naphthol Solution Protocol

Solution contains 0.48 mM 4-Chloro-1-Naphthol, 50 mM Tris-HCl and 0.2 M NaCl in 17% methanol. This is a substrate solution designed for visualizing horseradish peroxidase conjugates in Western Blotti...
Keywords: Western blot

Enzymatic Assay of Catalase (EC 1.11.1.6)

The continuous spectrophotometric rate reduction determination (A240, Light path = 1 cm) is based on the following reaction:
Keywords: Reductions

Enzymatic Assay of Peroxidase (EC 1.11.1.7) 2,2'-Azino-bis(3-Ethylbenzthiazoline-6-Sulfonic Acid) as a Substrate

Replaces SPABTS02. Procedure updated to conform to current QUMAS formatting guidelines. Refer to CR SOP-DEK-ENZ42.
Keywords: Extinction coefficient

Enzymatic Assay of Peroxidase (EC 1.11.1.7)

This procedure is for the determination of Peroxidase enzymatic activity using Pyrogallol as the substrate. Products using this method include, but are not limited to, P1709, P2088, P6140, P6782, P81...
Keywords: Enzyme activity, Extinction coefficient

Explant Sterilization - Plant Tissue Culture Protocol

Orchid seeds are very small and contain little to no food reserves. A single seed capsule may contain 1,500 to 3,000,000 seeds. Sowing the seed in vitro makes it possible to germinate immature seed (...
Keywords: Sterilizations

Peer-Reviewed Papers
15

References

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