H3375 Sigma


≥99.5% (titration)

Synonym: 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid, N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid)

  • CAS Number 7365-45-9

  • Empirical Formula (Hill Notation) C8H18N2O4S

  • Molecular Weight 238.30

  •  Beilstein Registry Number 883043

  •  EC Number 230-907-9

  •  MDL number MFCD00006158

  •  PubChem Substance ID 24895572



Related Categories Apoptosis and Cell Cycle, Biochemicals and Reagents, Biological Buffers, Buffers, Buffers A to Z,
Quality Level   ELITE
assay   ≥99.5% (titration)
pH   5.0-6.5 (25 °C, 238 g/L)
useful pH range   6.8 - 8.2
pKa (25 °C)   7.5
Featured Industry   Diagnostic Assay Manufacturing



1 kg in poly bottle

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5, 25, 50 kg in poly drum

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Safety Information

NONH for all modes of transport
WGK Germany 

Frequently Asked Questions

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What is the purpose of using HEPES in cell culture?
HEPES provides supplemental buffering for media in pH range of 7.2 to 7.6. It has no nutritional value to cells.
What is the concentration of HEPES used in cell culture?
HEPES is typically used in cell culture in the range of 10-25 mM.
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