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H3375 Sigma

HEPES

≥99.5% (titration)

Synonym: 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid, N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid)

  • CAS Number 7365-45-9

  • Empirical Formula (Hill Notation) C8H18N2O4S

  • Molecular Weight 238.30

  •  Beilstein Registry Number 883043

  •  EC Number 230-907-9

  •  MDL number MFCD00006158

  •  PubChem Substance ID 24895572

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Properties

Related Categories Apoptosis and Cell Cycle, Biochemicals and Reagents, Biological Buffers, Buffers, Buffers A to Z,
InChI Key   JKMHFZQWWAIEOD-UHFFFAOYSA-N
Quality Level   ELITE
assay   ≥99.5% (titration)
pH   5.0-6.5 (25 °C, 238 g/L)
useful pH range   6.8 - 8.2
pKa (25 °C)   7.5
Featured Industry   Diagnostic Assay Manufacturing

Description

Packaging

1 kg in poly bottle

25, 100, 250, 500 g in poly bottle

5, 25, 50 kg in poly drum

Legal Information

Redi-Dri is a trademark of Sigma-Aldrich Co. LLC

Other Notes

Go to Redi-Dri Product Listing RDD002

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
1
RTECS 
TL6809000

Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
How do I get lot-specific information or a Certificate of Analysis?
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
How do I find price and availability?
There are several ways to find pricing and availability for our products.Once you log onto our website, you will find the price and availability displayed on the product detail page.You can contact any of our Customer Sales and Service offices to receive a quote. USA customers: 1-800-325-3010 orview local office numbers.
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
What is the purpose of using HEPES in cell culture?
HEPES provides supplemental buffering for media in pH range of 7.2 to 7.6. It has no nutritional value to cells.
What is the concentration of HEPES used in cell culture?
HEPES is typically used in cell culture in the range of 10-25 mM.
My question is not addressed here, how can I contact Technical Service for assistance?
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Protein Stabilizer

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Protocols & Articles

Articles

3D Organoid Culture: New In Vitro Models of Development and Disease

Model systems drive biological research by recapitulating body processes and functions from the molecular to whole organism level. The human body is composed of both cellular and non-cellular materia...
Keywords: Adhesion, Cell attachment, Cell culture, Cryopreservation, Diseases, Growth factors, Infectious Diseases, Processes and Functions, Vitamins

Protocols

The use of differential scanning fluorimetry to detect ligand interactions that promote protein stability

Differential scanning fluorimetry (DSF) is a rapid and inexpensive screening method to identify low-molecular-weight ligands that bind and stabilize purified proteins. The temperature at which a prot...
Keywords: AGE, Absorption, Amplification, Biochemistry, Buffers, Calorimetry, Crystallization, Detergents, Drug discovery, Evaporation, Extinction coefficient, Gene expression, Genomics, Ligands, Melting, Nucleic acid denaturation, Phase transitions, Polymerase chain reaction, Polymerase chain reaction - quantitative, Precipitation, Purification, Sample preparations, Spectroscopy, Titrations

Related Content

BioUltra Biological Buffers

A buffer, as defined by Van Slyke [1], is "a substance which by its presence in solution increases the amount of acid or alkali that must be added to cause unit change in pH". Buffers are thus very i...
Keywords: Absorption, Adsorption, Biochemistry, Biological Buffers, Biological processes, Buffers, PAGE

Buffer Reference Center

Citric Acid – Na2HPO4 Buffer Solutions, pH approx. 2.6–7.61 Citric acid monohydrate, C6H8O7 • H2O, M. wt. 210.14; 0.1M-solution contains 21.01 g/l. Na2HPO4, M. wt. 141.98; 0.2M-solution contains 28.4...
Keywords: Biological Buffers, Buffers

缓冲液参考中心

柠檬酸 – Na2HPO4 缓冲液,pH约为 2.6–7.61 柠檬酸一水合物, C6H8O7 • H2O, M. wt. 210.14; 0.1M的溶液中含有21.01 g/l。 Na2HPO4, M. wt. 141.98; 0.2M的溶液中含有28.40g / l 或 Na2HPO4 • 2H2O, M. wt. 178.05; 0.2M的溶液中含有35.61 g/l。 xml 0.1M的...

Peer-Reviewed Papers
15

References

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Product #

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