H4034 Sigma


BioPerformance Certified, ≥99.5% (titration), cell culture tested

Synonym: 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid, N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid)

  • CAS Number 7365-45-9

  • Empirical Formula (Hill Notation) C8H18N2O4S

  • Molecular Weight 238.30

  •  Beilstein Registry Number 883043

  •  EC Number 230-907-9

  •  MDL number MFCD00006158

  •  PubChem Substance ID 24895616



Related Categories BioPerformance Certified Buffers, Biochemicals and Reagents, Biological Buffers, Buffer / Buffer Salts, Buffered Salt Solutions,
Quality Level   ELITE
grade   BioPerformance Certified
assay   ≥99.5% (titration)
impurities   endotoxin and total aerobic microbial count, tested
useful pH range   6.8 - 8.2
pKa (25 °C)   7.5
cation traces   Fe: ≤5 ppm
absorption   A0.1M/260, H2O ≤0.05
  A0.1M/280, H2O ≤0.05
suitability   cell culture tested
Featured Industry   Diagnostic Assay Manufacturing
foreign activity   DNase, RNase, NICKase, protease, none detected


Other Notes

Easily compare specifications for HEPES products with the HEPES specification table.


1 kg in poly bottle

100, 500 g in poly bottle

25 kg in poly drum


• HEPES has been used for the adjustment and maintenance of pH of biological solutions.
• It has been added to the culture medium of cells.
• It has been used for homogenization of tissue. It has also been used in the preparation of cytosolic and nuclear extract from cells.

General description

HEPES is a zwitterion buffer. HEPES buffer does not confer cytotoxic effects on cells and thus can be used in animal cell cultures.

Safety & Documentation

Safety Information

NONH for all modes of transport
WGK Germany 

High Quality GMP-Buffers
Protocols & Articles


Immunoprecipitation Procedure

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Keywords: Buffers, Cell disruption, Centrifugation, Enzyme activity, Immobilization, Immunoprecipitation, Individual protein Immunoprecipitation, PAGE, Size-exclusion chromatography, Western blot

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Buffer Reference Center

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