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H4890 Sigma-Aldrich

Anti-Human Hemoglobin antibody produced in rabbit

whole antiserum

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies to Human Plasma Proteins, H1-HE, Primary Antibodies,
conjugate   unconjugated
clone   polyclonal
biological source   rabbit
application(s)   indirect ELISA: 1:8,000
species reactivity   human
shipped in   dry ice
storage temp.   −20°C
antibody form   whole antiserum
Quality Level   200
antibody product type   primary antibodies
contains   15 mM sodium azide
UniProt accession no.   P68871
Gene Information   human ... HBB(3043)

Description

General description

HBB (hemoglobin subunit β) codes for a β globin that consists of three exons. This gene is located on human chromosome 11p15. HBB is a serum protein that belongs to the histone-like protein family.

Immunogen

Hemoglobin isolated from normal adult human red blood cells and treated to remove lipoproteins.

Application

Anti-Human Hemoglobin antibody has been used in pulse chase experiments and western blotting.

Anti-Human Hemoglobin antibody produced in rabbit was used for immunoblotting using crude human cerebellar protein extracts and membrane extracts of human RBC. It was used for immunohistochemistry of human tongue cancer samples.

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Biochem/physiol Actions

HBB (hemoglobin subunit β) participates in oxygen transport from the lung to various peripheral tissues. Mutation in HBB result in Sickle cell disease (SCD). Overexpression of HBB has been observed in patients with periodontal disease.

Hemoglobin (Hb) is oxygen-binding metalloprotein that is present in the red blood cells. The most abundant Hb is HbA tetramer that is made up of two α, two β chains and the nonprotein heme group. The heme group contains iron ion and a porphyrin ring. Mutations in Hb gene result in hemoglobinopathies, the best known being sickle-cell disease and thalassemias.

Preparation Note

treated to remove lipoproteins

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 3
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

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Peer-Reviewed Papers
15

References

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