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H8264 Sigma-Aldrich

Hanks’ Balanced Salt solution

Modified, with sodium bicarbonate, without phenol red, liquid, sterile-filtered, suitable for cell culture

  •  NACRES NA.75



Related Categories Balanced Salt Mixtures and Solutions, Cell Culture, Classical Media and Salts, Hank’s Balanced Salts
Quality Level   300
sterility   sterile-filtered
form   liquid
application(s)   cell culture | mammalian: suitable
impurities   endotoxin, tested
components   NaHCO3: 0.35 g/L
glucose: 1.0 g/L (Dextro)
phenol red: no
shipped in   ambient


General description

Hanks’ Balanced Salt solution (HBSS) is a buffer that mimics biological medium and stabilizes cells. It is useful in leucocyte labeling studies. HBSS is an effective preservative for periodontal ligament cells. HBSS lacks D-glucose and essential inorganic ions and is useful in starvation studies.


Hanks’ Balanced Salt solution has been used:
• as a rinse solution for hepatocytes
• for hippocampi dissection
• in corrosion testing of dog-bone specimens
• for starvation studies in breast cancer cell line
• as a component of dissociation solution for dorsal root ganglion
• as a chamber solution for the patch-clamp apparatus

Other Notes

Phenol red has been shown to interfere with the growth of some cells at cloning densities. Use this medium when working with stem cells or when growing cells at low densities.

Safety & Documentation

Safety Information

NONH for all modes of transport
WGK Germany 
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable
Protocols & Articles


Detection of Endogenous Pathway Activity in Novel Reporter Cell Lines

CompoZr® ZFN (zinc finger nuclease) technology was used to insert the sequence for fluorescent proteins adjacent to endogenous genes for signal transduction proteins to create novel reporter cell lin...
Andrey Samsonov, Senior Scientist; Hongyi Zhang, Senior Scientist; Fan Zhang, Senior Scientist; Nathan Zenser, Senior Scientist; John Fetter, Associate Research Fellow; Dmitry Malkov, Principal Investigator
Biowire Spring 2012, 13–18
Keywords: Angiogenesis, Antitumor agents, Apoptosis, Cell signaling, Gene expression, Growth factors, Immunostaining, Ligands, Recombination, Transcription, Transduction

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Cell Culture Troubleshooting Guide

Cell culture has become one of the most fundamental techniques for modeling biological systems, and is of increasing importance in the biotechnology and pharmaceutical sectors as well as an essential...
Keywords: Antibiotics, Apoptosis, Cell attachment, Cell culture, Cryopreservation, Filtration, Growth factors, Pharmaceutical, Phase transitions

Peer-Reviewed Papers


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