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H9268 Sigma

Hexadimethrine bromide

≥94% (titration)

Synonym: 1,5-Dimethyl-1,5-diazaundecamethylene polymethobromide, Polybrene

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Properties

Related Categories Core Bioreagents, General Reagents, Life Science Reagents for RNAi, Life Science Reagents for Transfection, Research Essentials More...
assay   ≥94% (titration)
storage temp.   2-8°C

Description

Packaging

5, 10, 50, 100 g in poly bottle

Application

Hexadimethrine bromide can be used to transfect mammalian cells with DNA. It can be used to increase the efficiency of lipofection transfections.
Hexadimethrine bromide has been used for lentivirus infection in cells. It has been used for infection of cells with retroviral supernatant.

General description

Hexadimethrine bromide is a quaternary ammonium compound which works as a heparin antagonist. It is also involved in the transformation of low molecular weight DNA.

Safety & Documentation

Safety Information

Symbol 
GHS07  GHS07
Signal word 
Warning
Hazard statements 
RIDADR 
NONH for all modes of transport
WGK Germany 
3
RTECS 
TQ2655000

Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
IsProduct H9268, Hexadimethrine bromide, the same thing as Polybrene?
Yes, Polybrene (registered trade-mark of Abbott) and 1,5-Dimethyl-1,5-diazaundecamethylene polymethobromide, are Synonyms.
What is the molecular weight of Product H9268, Hexadimethrine bromide?
According to the information from our supplier, the molecular weightof this product is4,000-6,000. The Merck Index provides a general MW rangefor the chemical of 5,000 - 10,000.
What is the solubility of Product H9268, Hexadimethrine bromide?
Soluble in water up to 10%.
How do I sterilize Product H9268, Hexadimethrine bromide?
Hexadimethrine bromide can be filtered sterilized or autoclaved.
What is the solution stability of Product H9268, Hexadimethrine bromide?
Stock solutions in 0.9% saline solution at 1 mg/mL are stable at least one year stored in the refrigerator. Stability information is found on the Product Bulletin.
How do I get lot-specific information or a Certificate of Analysis?
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
How do I find price and availability?
There are several ways to find pricing and availability for our products.Once you log onto our website, you will find the price and availability displayed on the product detail page.You can contact any of our Customer Sales and Service offices to receive a quote. USA customers: 1-800-325-3010 orview local office numbers.
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
My question is not addressed here, how can I contact Technical Service for assistance?
Ask a Scientist here.
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Protocols & Articles

Articles

Examination of Efficacy, Specificity, and Efficiency of an shRNA Lentiviral Library

Betsy Boedeker,* Gregory A. Wemhoff, Andrea Spencer, Roger Chiu, Suzanne Miller, Henry George, and Edward J. Weinstein
Betsy Boedeker, Gregory A. Wemhoff, Andrea Spencer,Roger Chiu, Suzanne Miller, Henry George and Edward J. Weinstein
LSI Volume 7 Article 1
Keywords: Amplification, Antibiotics, Cellular processes, Drug discovery, Functional genomics, Gene expression, Genomics, Molecular biology, Nucleic acid denaturation, Polymerase chain reaction, Polymerase chain reaction - quantitative, Reductions, Sequences, Transcription, Transduction, Vectors

Safe Handling of Lentivirus

Our lentiviral vector systems have been developed with enhanced safety features. Numerous precautions are in place in the design of our lentiviruses to prevent replication, including eliminating viru...
Keywords: Environmental, Genetic, Recombination, Transfection

Protocols

CRISPR Lentiviral Screening: Antibiotic Selection

A kill curve is a dose-response experiment where cells are subjected to a range of antibiotic concentrations to determine the minimum concentration of antibiotic needed to kill all cells over a desir...
Keywords: Antibiotics, Apoptosis, Gene expression, Transduction

Lentiviral Transduction Protocol

Prepare mammalian cells so that they are growing exponentially and are no more than 70-80% confluent before transduction.
Keywords: Cell culture, Gene expression, Molecular biology, Transduction

Protocol, FACS Titration of Lentivirus

FACS Analysis of CFU FACS (Fluorescence-Activated Cell Sorting) provides a method for sorting a mixed population of cells into two or more groups, one cell at a time, based on the specific light scat...
Keywords: Enzyme-linked immunosorbent assay, Flow cytometry, Gene expression, Titrations, Transduction

Successful Transduction Using Lentivirus

Successful targeting relies on optimizing key sensitive steps in the process, including lentiviral transduction. Below are some helpful handling and titration tips from our R&D lentiviral experts. As...
Keywords: Antibiotics, Enzyme-linked immunosorbent assay, Gene expression, PAGE, Polymerase chain reaction, Titrations, Transduction

Related Content

MISSION® LentiPlex® Pooled shRNA Library Screening in Mammalian Cells [VIDEO]

The MISSION LentiPlex Human shRNA Pooled Library is a genome-wide lentiviral pool produced using a proprietary process. The TRC 1.0 library consists of over 80,000 shRNA constructs from the TRC colle...
Keywords: Amplification, Antitumor agents, Cancer, Oncology, Sequencing

shRNA慢病毒文库的功效、特异性和效率检验

Betsy Boedeker,* Gregory A. Wemhoff, Andrea Spencer, Roger Chiu, Suzanne Miller, Henry George, and Edward J. Weinstein
Keywords: Functional genomics, Genomics, Polymerase chain reaction, Transduction

Peer-Reviewed Papers
15

References

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