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I2911 Sigma-Aldrich

Iscove’s Modified Dulbecco’s Medium

AQmedia, With L-alanyl-L-glutamine and sodium bicarbonate, sterile-filtered, liquid, suitable for cell culture

Synonym: IMDM

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Properties

Related Categories Cell Culture, Cell Culture Media, Core Bioreagents, Life Science Reagents for Cell Culture, Research Essentials,
grade   AQmedia
sterility   sterile-filtered
form   liquid
application(s)   cell culture | hybridoma: suitable
  cell culture | mammalian: suitable
impurities   Endotoxin, tested
components   glucose: yes
phenol red: yes
stable glutamine: yes
NaHCO3: yes
shipped in   ambient
storage temp.   2-8°C

Description

General description

This IMDM differs from the original formula, because it is supplemented with L-alanyl-L-glutamine dipeptide. Glutamine is notoriously unstable in cell culture. This dipeptide provides a more stable form of glutamine for use in cell cultures.

Application

Guilbert and Iscove demonstrated that precursor cells of erythrocytes and macrophages could be cultured in a totally defined serum free medium supplemented with albumin, transferrin, lecithin, and selenium. This medium is a modification of Dulbecco′s Modified Eagle′s Medium (DME) containing selenium, additional amino acids and vitamins, sodium pyruvate, HEPES buffer, and potassium nitrate instead of ferric nitrate. Further studies demonstrated that Iscove′s Medium would support murine B lymphocytes, hemopoietic tissue from bone marrow, B cells stimulated with lipopolysaccharide, T lymphocytes, and a variety of hybrid cells.

Linkage

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Legal Information

AQmedia is a trademark of Sigma-Aldrich Co. LLC

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
1

Single- & multi-channel pipettes from BRAND

Pipette Controller from Brand
Protocols & Articles

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Peer-Reviewed Papers
15

References

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