EMAIL THIS PAGE TO A FRIEND
I4131 Sigma-Aldrich

IgG from rat serum

reagent grade, ≥95% (SDS-PAGE), essentially salt-free, lyophilized powder

Synonym: Rat IgG

Purchase

Properties

Related Categories Antibodies, Normal Human and Animal Immunoglobulins, Purified Immunoglobulins, Sera and Immunoglobulins, Supplementary Products More...
Quality Level   200
grade   reagent grade
assay   ≥95% (SDS-PAGE)
form   essentially salt-free, lyophilized powder
conjugate   unconjugated
storage temp.   2-8°C

Description

General description

IgG antibody provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defence of the neonate against infections Rat IgG is purified from pooled normal rat serum by fractionation and ion-exchange chromatography.

IgG antibody subtype is the most abundant serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids, Immunoglobins have two heavy (H) and two light (L) chains, linked by disulphide linkages. Each light chain comprises one variable N-terminal region and a constant C-terminal region. Heavy chain has one variable N-terminal region and three or four constant (CH1-CH4) C-terminal region. The four classes of IgG can be IgG1, IgG2, IgG3, and IgG4.

Application

IgG from rat serum has been used:
•  in immunolabeling
• in flow cytometry
• in enzyme linked immunosorbent assay
• as a control in neutrophil depletion assay

Packaging

10, 50, 100 mg in poly bottle

Biochem/physiol Actions

IgG antibody have enormous therapeutic potential and its fragment crystallizable (Fc) region contributes for the development of therapeutic antibody.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety & Documentation

Safety Information

Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 3
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

Please Enter a Lot Number

Certificate of Origin (COO)

Please Enter a Lot Number
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Imprint RIP | uRNA Quantitation | MicroRNA primers | MicroRNA Reverse Transcription

The Imprint® RNA Immunoprecipitation Kit was used to co-purify human argonaute 2 (Ago2)-associated RNAs from HeLa cells, as idicated in the Technical Bulletin. To evaluate the different stringency op...
Carol Kreader
Principal R&D Scientist
Keywords: Buffers, Cell disruption, Immunoprecipitation, Polymerase chain reaction - quantitative, RNA immunoprecipitation, Western blot

Protocol for Anti Ago-RNA Immunoprecipitation from mammalian cells using the RIP kit

1.    Grow cells to ~80% confluency.  You will need ~2 million cells/RIP, or ~4 million cells for IgG (neg control) & Ago2 RIP
Keywords: Cell disruption, Immunoprecipitation, Individual protein Immunoprecipitation, RNA immunoprecipitation

Related Content

Antibody Explorer | Buy Primary Antibodies & Secondary Antibodies

Primary, Secondary and Recombinant Monoclonal Antibodies Providing highly cited primary and secondary antibodies, we have you covered for your ELISA, western blot, immunohistochemistry or other assay...
Keywords: Alzheimer Disease, Cancer, Chromatin immunoprecipitation, Diagnostic, Enzyme-linked immunosorbent assay, Flow cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Neuroscience, Western blot

Peer-Reviewed Papers
15

References

Related Products

related product

Product #

Description

Add to Cart

08168 Timestrip Plus 8 °C

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?