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L4391 Sigma-Aldrich

Lipopolysaccharides from Escherichia coli O111:B4

γ-irradiated, BioXtra, suitable for cell culture

Synonym: LPS

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Properties

Related Categories Bacteria-derived adjuvants, Biochemicals and Reagents, Cell Biology, Cell Culture, Cell Signaling and Neuroscience,
Quality Level   300
sterility   γ-irradiated
product line   BioXtra
form   lyophilized powder
purified by   gel-filtration chromatography
storage condition   desiccated
application(s)   cell culture | mammalian: suitable
impurities   <1% Protein (Lowry)
solubility   H2O: 5 mg/mL, slightly hazy
storage temp.   2-8°C

Description

Application

This LPS serotype has been used to stimulate B-cells and induce NOS in human hepatocytes.

Lipopolysaccharides (LPSs) are characteristic components of the cell wall of Gram-negative bacteria. LPS and its lipid A moiety stimulate cells of the innate immune system by the Toll-like receptor 4 (TLR4), a member of the Toll-like receptor protein family, which recognizes common pathogen-associated molecular-patterns (PAMPs).

Packaging

1 mg in glass bottle

Biochem/physiol Actions

LPS is a major constituent of the cell wall of most gram negative bacteria. It is a highly immunogenic antigen with the ability to enhance immune responses to soluble antigens. LPS also acts as a specific mitogen for bone marrow derived B lymphocytes from mice, rabbits, chickens, cows, hamsters, and humans.

Reconstitution

Lipopolysaccharides are supplied as lyophilized, γ-irradiated powders. To reconstitute, add 1 ml sterile balanced salt solution or tissue culture medium to the vial (1 mg) and gently swirl until the powder dissolves. Reconstituted product may be further diluted to desired working concentrations using sterile balanced salt solution or tissue culture medium.

Safety & Documentation

Safety Information

Symbol 
GHS06  GHS06
Signal word 
Danger
Hazard statements 
Precautionary statements 
RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 3
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Certificate of Origin (COO)

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pHast Pack
Protocols & Articles

Articles

Choosing the optimal vaccine adjuvant

The word "vaccination" was first coined by English scientist Edward Jenner in 1796, although early forms of vaccination have been documented as far back as the 17th century.1 Vaccination is used to g...
BioFiles Vol. 5, No. 3
Keywords: Cancer, Clinical, Diseases, Inflammation, Veterinary

Human Microbiome Research Workflow: An Overview of the Role in Human Health and Disease

Previously, the study of human microbiology was based on identification, isolation and culture of single microbes, such as bacteria, fungi and viruses. Scientists collected these organisms from patie...
Keywords: Amplification, Antibiotics, Antifungals, Cell culture, Cell disruption, Clinical, Diabetes, Digestions, Diseases, Environmental, Gas chromatography, Gas chromatography mass spectrometry, Gastrointestinal, Genetics, Genomics, High performance liquid chromatography, Liquid chromatography mass spectrometry, Mass spectrometry, Metabolites, Metabolomics, Microbiology, Nutrition, Obesity, Polymerization reactions, Proteomics, Respiratory, Sample preparations, Separation, Sequencing

Protocols

Lipopolysaccharides (LPS)

Lipopolysaccharides (LPS) are characteristic components of the cell wall of Gram negative bacteria; they are not found in Gram positive bacteria. They are localized in the outer layer of the membrane...
Keywords: Adsorption, Antibiotics, Cell culture, Cell disruption, Chromatography, Culture media, Filtration, Immunology, Ion Exchange, Metabolism, Positron Emission Tomography, Purification, Size-exclusion chromatography, Solvents

Related Content

Cell Culture Troubleshooting Guide

Cell culture has become one of the most fundamental techniques for modeling biological systems, and is of increasing importance in the biotechnology and pharmaceutical sectors as well as an essential...
Keywords: Antibiotics, Apoptosis, Cell attachment, Cell culture, Cryopreservation, Filtration, Growth factors, Pharmaceutical, Phase transitions

Peer-Reviewed Papers
15

References

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