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M2933 Sigma-Aldrich

MES hydrate

BioPerformance Certified, suitable for cell culture, ≥99.5%

Synonym: 2-(N-Morpholino)ethanesulfonic acid hydrate, 4-Morpholineethanesulfonic acid

  • CAS Number 1266615-59-1

  • Empirical Formula (Hill Notation) C6H13NO4S · xH2O

  • Molecular Weight 195.24 (anhydrous basis)

  •  MDL number MFCD00149409

  •  eCl@ss 32129211

  •  PubChem Substance ID 329817955

  •  NACRES NA.25

Purchase

Properties

Related Categories BioPerformance Certified Buffers, Biochemicals and Reagents, Biological Buffers, Buffers / Buffer salts, Buffers / Buffers Salts,
Quality Level   400
grade   BioPerformance Certified
  for molecular biology
assay   ≥99.5%
application(s)   cell culture | mammalian: suitable
impurities   endotoxin and Total Aerobic Microbial Count, tested
useful pH range   5.5 - 6.7
pKa    6.1
Featured Industry   Diagnostic Assay Manufacturing
foreign activity   DNase, RNase, protease, none detected
SMILES string   O.OS(=O)(=O)CCN1CCOCC1
InChI   1S/C6H13NO4S.H2O/c8-12(9,10)6-3-7-1-4-11-5-2-7;/h1-6H2,(H,8,9,10);1H2
InChI key   MIIIXQJBDGSIKL-UHFFFAOYSA-N

Description

General description

MES is one of the Good′s buffers, the most extensively used biological buffers. MES is a zwitterionic N-substituted aminosulfonic acid with a morpholinic ring. It does not form complexes with majority of the metals used in environmental and biological studies. It is easily soluble in water and has minimum lipid solubility, making it impermeable to membranes.

Application

MES Hydrate has been used:
• To suspend cells before autophagic induction studies
• In the preparation of Murashige and Skoog growth medium for the growth of Arabidopsis thaliana seedlings
• In the conjugation of hybridization probes to beads before PCR amplification
• To treat fibroblast-derived matrix before conjugation with heparin for use as a vascular endothelial growth factor delivery platform

Packaging

1 kg in poly bottle

25, 100, 500 g in poly bottle

Preparation Note

A buffer using MES can be prepared by titrating with NaOH to the desired pH. Alternatively, stock solutions of MES and MES sodium salt can be mixed to attain the desired pH. Standard mixing tables using stock solutions to prepare buffers of a given pH have been published. MES is not recommended for buffering at pH 7.4; other buffers should be considered.

Storage and Stability

Solutions are stable at 2-8°C for months. Sterilize by filtration through 0.2uM filters. Autoclaving is not recommended for any sulfonic acid buffer. If buffers must be nuclease-free, treat the water first, and then add the buffer after autoclaving. When MES solutions are autoclaved, they turn yellow (although pH does not change measurably). The identity of the yellow breakdown product is unknown.

Other Notes

Easily compare specifications for MES products with the MES specification table.

Solubility: MES is soluble in water, giving a clear colourless solution at concentrations of 0.5 M or higher. Similar product MES hydrate M8250 is tested at 20g/80mL water, or 1.3M. The pH of a solution should be between 2.5 and 5, depending on the concentration. A saturated solution at 0°C is approximately 0.65M.

Safety & Documentation

Safety Information

Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
3

pHast Pack
Protocols & Articles

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