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M3546 Sigma-Aldrich

Ultra-low Range Molecular Weight Marker (M.W. 1,060-26,600)

  •  NACRES NA.25

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Properties

Related Categories Molecular Biology, Molecular Weight Markers, Protein Electrophoresis, Proteomics
Quality Level   100
  200
usage    vial sufficient for 400 applications on large gels (16 × 18 cm)
   vial sufficient for 800 mini-gels (10 × 10 cm)
packaging   vial of 200 μL
storage temp.   −20°C

Description

Application

Ultra-low Range Molecular Weight Marker (M.W. 1,060-26,600) has been used as a protein marker in sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and western blot.

Biochem/physiol Actions

The ultralow range marker is designed for use in the Tris-Tricine SDS-PAGE method when stained with Brilliant blue G (non-methanol based). Glutaraldehyde fixing is recommended with the ultralow marker in order to keep the two smallest proteins fixed in the gel. The ultralow range marker is supplied as a liquid, which must be diluted (1:20) with the supplied sample buffer prior to loading.

Physical form

Solution in 0.1 M Tris-HCl, pH approx. 8.5, with 4 mM EDTA, 3 mM sodium azide and 40% glycerol

Safety & Documentation

Safety Information

Symbol 
Signal word 
Danger
Hazard statements 
Precautionary statements 
RIDADR 
UN 3082 9 / PGIII
WGK Germany 
WGK 3
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable
Protocols & Articles

Articles

HPLC Analysis of Various Cationic Polymers on TSKgel® PWXL-CP Columns

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Various Cationic Polymers on TSKgel® PWXL-CP Columns
Keywords: Chromatography, High performance liquid chromatography

Related Content

Molecular Weight Markers

Protein molecular weight markers, sometimes identified as protein standards or protein ladders, are used to estimate the molecular weight of proteins of interest, to monitor the progress of electroph...
Keywords: Electrophoresis, Gel electrophoresis, Isoelectric focusing, Molecular biology, PAGE, Polymerase chain reaction, Protein electrophoresis, Separation, Western blot

Peer-Reviewed Papers
15

References

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