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M3769 Sigma-Aldrich

Medium 199

Modified, with Earle′s salts, without L-glutamine, sodium bicarbonate, and phenol red, powder, suitable for cell culture

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Properties

Related Categories Cell Culture, Cell Culture Media, Classical Media and Salts, Core Bioreagents, Life Science Reagents for Cell Culture,
form   powder
application(s)   cell culture | mammalian: suitable
components   phenol red: no
HEPES: no
sodium pyruvate: no
L-glutamine: no
NaHCO3: no
Earle’s salts (5% CO2): yes
shipped in   ambient
storage temp.   2-8°C

Description

Application

Originally developed as a completely defined media formulation for the culture of primary explants. These media have broad applicability, particularly for non-transformed cells and are widely used for vaccine production, primary pancreatic explants, and lens tissues.

Quantity

Formulated to contain 9.4 grams of powder per liter of medium.

Reconstitution

Supplement with 0.1 g/L L-glutamine, 2.2 g/L sodium bicarbonate.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
1

Documents

Certificate of Analysis (COA)

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Protocols & Articles

Protocols

Powdered Media Preparation Instructions

Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture. The entire contents of each package should be used immediately after opening. Preparing m...

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Cell Culture Troubleshooting Guide

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Peer-Reviewed Papers
15

References

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