• USA Home
  • M5650 - Minimum Essential Medium Eagle

EMAIL THIS PAGE TO A FRIEND
M5650 Sigma-Aldrich

Minimum Essential Medium Eagle

With Earle′s salts, non-essential amino acids and sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture

Synonym: MEM

  •  NACRES NA.75

Purchase

Properties

Related Categories Cell Culture, Cell Culture Media, Classical Media and Salts, Core Bioreagents, Life Science Reagents for Cell Culture,
sterility   sterile-filtered
form   liquid
application(s)   cell culture | mammalian: suitable
impurities   endotoxin, tested
components   Earle’s salts (5% CO2): yes
L-glutamine: no
sodium pyruvate: no
NaHCO3: yes
phenol red: yes
HEPES: no
shipped in   ambient
storage temp.   2-8°C

Description

Application

Contains 19 amino acids. The essential amino acids and the non-essential amino acids; L-ala; L-asn; L-asp; L-glu; L-gly; L-pro and L-ser. May be preferred for growth of adherent cells in the presence of reduced serum or as a base for development of a serum free medium.

Reconstitution

Supplement with 0.292 g/L L-glutamine.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

Single- & multi-channel pipettes from BRAND

Pipette Controller from Brand
Protocols & Articles

Related Content

Cell Culture Troubleshooting Guide

Cell culture has become one of the most fundamental techniques for modeling biological systems, and is of increasing importance in the biotechnology and pharmaceutical sectors as well as an essential...
Keywords: Antibiotics, Apoptosis, Cell attachment, Cell culture, Cryopreservation, Filtration, Growth factors, Pharmaceutical, Phase transitions

MEM (Minimum Essential Medium Eagle)

Minimum Essential Medium (MEM), developed by Harry Eagle, is one of the most widely used of all synthetic cell culture media. Early attempts to cultivate normal mammalian fibroblasts and certain subt...
Keywords: Addition reactions, Cell culture, Culture media, Eliminations

Peer-Reviewed Papers
15

References

Related Products

related product

Product #

Description

Add to Cart

F2442 Fetal Bovine Serum, USA origin, sterile-filtered, suitable for cell culture, suitable for hybridoma
08168 Timestrip Plus 8 °C

supplement

Product #

Description

Add to Cart

G7513 L-Glutamine solution, 200 mM, solution, sterile-filtered, BioXtra, suitable for cell culture

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?