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M8403 SAFC

McCoy’s 5A Medium

Modified, with sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture

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Properties

Related Categories Cell Culture, Cell Culture Media, Classical Media and Salts, Core Bioreagents, Life Science Reagents for Cell Culture,
Quality Level   500
sterility   sterile-filtered
form   liquid
application(s)   cell culture | mammalian: suitable
impurities   endotoxin, tested
components   NaHCO3: 2.2  g/L
phenol red: 0.011 g/L
L-glutamine: no
glucose: 3.0 g/L
glucose: 3 g/L
shipped in   ambient
storage temp.   2-8°C

Description

Application

Used for the primary culture of cells from a wide range of tissues. Recommend for production of virus in primary cell cultures.

Biochem/physiol Actions

Originally developed to support liver tumor cells by modification of the amino acids found in BME. This formulation has also be used in the culture of bone marrow, skin, gingiva, kidney, omentum, adrenals, lung, spleen, rat embryos, and other cell types.

Reconstitution

Supplement with 0.22 g/L L-glutamine.

Safety & Documentation

Safety Information

Symbol 
GHS07  GHS07
Signal word 
Warning
Hazard statements 
Precautionary statements 
RIDADR 
NONH for all modes of transport
WGK Germany 
1

Pipette Controller from Brand
Protocols & Articles

Articles

Long-term, live cell analysis of host-pathogen interactions using the CellASIC® ONIX System

Host-pathogen interactions represent a signifcant area of biomedical research, encompassing the study of how viruses, bacteria, and other organisms cause infections and diseases or alter normal physi...
Keywords: Apoptosis, Cell culture, Diffusion, Diseases, Gene expression

Protocols

Cell Culture Protocol 10: Testing Cells for Mycoplasma Contamination by Hoechst DNA Staining

DNA staining methods such as indirect Hoechst staining techniques are quick with results available within 72 hours which compares favorably with 4 weeks for mycoplasma detection by culture isolation....
ECACC Laboratory Handbook 4th Edition
Keywords: Cell culture, Culture media, Detection methods, Indicators, Polymerase chain reaction

Cell Culture Protocol 2: Thawing of Frozen Cell Lines

Many cultures obtained from a culture collection, such as ECACC, will arrive frozen and in order to use the cells they must be thawed and put into culture. It is vital to thaw cells correctly in orde...
ECACC Laboratory Handbook 4th Edition
Keywords: Cell culture, Centrifugation, Culture media

Cell Culture Protocol 3: Subculture of Adherent Cell Lines

Adherent cell lines will grow in vitro until they have covered the surface area available or the medium is depleted of nutrients. At this point the cell lines should be subcultured or passaged in ord...
ECACC Laboratory Handbook 4th Edition
Keywords: Adhesion, Cell culture, Culture media

Cell Culture Protocol 4: Subculture of Semi-Adherent Cell Lines

Some cell lines grow as a mixed population (e.g. B95-8 - marmoset) where a proportion of cells do not attach to the tissue culture flask and remain in suspension. Therefore, to maintain this heteroge...
ECACC Laboratory Handbook 4th Edition
Keywords: Cell culture, Culture media

Cell Culture Protocol 5: Subculture of Suspension Cell Lines

In general terms, cell lines derived from blood (e.g. lymphocytes) grow in suspension cultures. Cells may grow as single cells or in clumps (e.g. EBV transformed lymphoblastic cell lines). For these ...
ECACC Laboratory Handbook 4th Edition
Keywords: Cell culture, Centrifugation, Culture media, Growth factors

Cell Culture Protocol 6: Cell Counting Using a Hemocytometer

For the majority of manipulations using cell lines, such as transfections, cell fusion techniques, cryopreservation and subculture routines it is necessary to quantify the number of cells prior to us...
ECACC Laboratory Handbook 4th Edition
Keywords: Carcinogens, Cell culture, Cryopreservation, Culture media, Flow cytometry

Related Content

Cell Culture Troubleshooting Guide

Cell culture has become one of the most fundamental techniques for modeling biological systems, and is of increasing importance in the biotechnology and pharmaceutical sectors as well as an essential...
Keywords: Antibiotics, Apoptosis, Cell attachment, Cell culture, Cryopreservation, Filtration, Growth factors, Pharmaceutical, Phase transitions

Peer-Reviewed Papers
15

References

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