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MAK092 Sigma-Aldrich

Peroxidase Activity Assay Kit

sufficient for 100 colorimetric or fluorometric tests

  •  NACRES NA.84

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Properties

Related Categories Cell Biology, Enzymatic Activity Assay Kits, Enzymatic Kits for the Quantitation of Nutrients and Metabolites, Metabolism Assay Kits A-Z, Metabolomics,
usage   sufficient for 100 colorimetric or fluorometric tests
storage temp.   −20°C

Description

General description

Peroxidase (EC 1.11.1.7) is an enzyme found broadly in biological systems that utilizes hydrogen peroxide in the oxidation of various substrates. The Peroxidase Activity Assay Kit provides a simple and direct procedure for measuring peroxidase activity in a variety of biological samples.

Application

Peroxidase Activity Assay Kit has been used to quantify the activity of peroxidase.

Features and Benefits

Compatible with high-throughput handling systems.

Suitability

Suitable for the determination of peroxidase activity in cell culture supernatant, serum, plasma, urine, and other biological fluids.

Principle

Peroxidase catalyzes the reaction between H2O2 and the probe, resulting in a colorimetric (570 nm)/fluorometric (λex = 535/λem = 587 nm) product, proportional to the peroxidase activity present. One unit of peroxidase is defined as the amount of enzyme that reduces1.0 μmole of H2O2 per minute at 37°C.

Safety & Documentation

Safety Information

RIDADR 
NA 1993 / PGIII
Flash Point(F) 
188.6 °F - closed cup
Flash Point(C) 
87 °C - closed cup
Protocols & Articles
Peer-Reviewed Papers
15

References

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