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MAK094 Sigma-Aldrich

Protein Carbonyl Content Assay Kit

sufficient for 100 colorimetric tests

  •  NACRES NA.84

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Description

General description

Oxidative stress results when the effectiveness of antioxidant defenses is insufficient to deal with the production of reactive oxygen species (ROS). ROS can induce damage to DNA, lipids, and proteins. The oxidation of proteins results in the production of stable carbonyl groups, which can be used as a measure of oxidative injury.

Application

Protein carbonyl content assay kit has been used to determine protein carbonyl concentration.

Suitability

Suitable for quantifying carbonyls in protein samples

Principle

The Protein Carbonyl Content Assay Kit provides a simple and direct procedure for measuring carbonyl content in a variety of biological samples. Carbonyl content is determined by the derivatization of protein carbonyl groups with 2,4-dinitrophenylhydrazine (DNPH) leading to the formation of stable dinitrophenyl (DNP) hydrazone adducts, which can be detected spectrophotometrically at 375 nm, proportional to the carbonyls present.

Safety & Documentation

Safety Information

Symbol 
Signal word 
Danger
Hazard statements 
Target organs 
Respiratory system
RIDADR 
UN 3316 9
Protocols & Articles
Peer-Reviewed Papers
15

References

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