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MAK157 Sigma-Aldrich

Renin Assay Kit

sufficient for 100 fluorometric tests

  •  NACRES NA.84

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Properties

Related Categories Assay Kits by Specialty Area, Cardiology - Cardiovascular, Cell Biology, Enzymatic Kits for the Quantitation of Nutrients and Metabolites, Metabolism Assay Kits A-Z,
usage   sufficient for 100 fluorometric tests
storage temp.   −20°C
Gene Information   human ... REN(5972)
rat ... REN(24715)

Description

General description

Renin, also known as angiotensinogenase, is an aspartyl protease that cleaves angiotensinogen to form angiotensin I, the initial and rate-limiting step of the renin-angiotensin system (RAS). This system plays a critical role in the regulation of blood pressure and fluid balance. Increased renin activity can result in hypertension due to increased vasoconstriction and fluid retention. Inhibitors of renin can be used as a treatment for hypertension.

Application

Renin Assay Kit has been used to determine the activity of renin.

Suitability

The Renin Assay Kit provides a convenient and simple method for screening renin activity in a variety of samples or for screening renin inhibitors.

Principle

Renin activity is determined using a proprietary fluorescence resonance energy transfer (FRET) peptide containing a fluorescence quencher. Cleavage of the peptide by renin results in the production of a fluorescent product (λex = 540/λem = 590 nm). This assay is ~50-fold more sensitive than the EDANS/DABCYL-based assay.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable
Protocols & Articles
Peer-Reviewed Papers
15

References

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