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MHS1 Sigma-Aldrich

Hematoxylin Solution, Mayer’s



Related Categories Cell Biology, Core Bioreagents, Hematology and Histology, Life Science Reagents for Immunohistochemistry (IHC), Research Essentials,
Quality Level   500
form   solution
shelf life   Expiry date on the label.
IVD   for in vitro diagnostic use
concentration   1 g/L
application(s)   hematology: suitable
  histology: suitable
pH   2.4 (25 °C)
storage temp.   room temp
SMILES string   Oc1cc2C[C@@]3(O)COc4c(O)c(O)ccc4[C@H]3c2cc1O
InChI   1S/C16H14O6/c17-10-2-1-8-13-9-4-12(19)11(18)3-7(9)5-16(13,21)6-22-15(8)14(10)20/h1-4,13,17-21H,5-6H2/t13-,16+/m0/s1


General description

Used as a counterstain for procedures such as immunohistochemistry or laser microdissection.


General purpose nuclear stain, progressive type. Used with hematoxylin and eosin staining.

Other Notes

1 g/L certified hematoxylin

Safety & Documentation

Safety Information

GHS07  GHS07
Signal word 
Hazard statements 
Precautionary statements 
NONH for all modes of transport
WGK Germany 
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable


Certificate of Analysis (COA)

Please Enter a Lot Number

Certificate of Origin (COO)

Please Enter a Lot Number

Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
How do I get lot-specific information or a Certificate of Analysis?
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
How do I find price and availability?
There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote. USA customers: 1-800-325-3010 or view local office numbers.
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
The website states that Mayer's Hematoxylin solution is a progressive type hematoxylin. What is a progressive type hematoxylin?
Hematoxylin solutions are generally classified as either progressive or regressive. Progressive formulations will stain only the nucleus. No staining should occur within the cytoplasm. Regressive hematoxylins have higher dye contents and if used for sufficient periods of time will overstain both the nucleus and the cytoplasm. Acid differentiation is required to remove the excessive stain from the cytoplasm and nucleus. This differentiation take practice to master. Too much or too little stain can be removed during differentiation, resulting in poorly stained slides. For users lacking extensive experience with the Hematoxylin and Eosin staining procedure,progressive hematoxylin formulations are often the better choice.
There are several Mayer's Hematoxylins listed on the website, beginning with the letters MHS.What are the differences?
Products MHS1, MHS16, MHS32, MHS80 and MHS128 are in fact the same product. In this case a unique product number was assigned to each package size. Other than the package size, there are no differences in formulation or use.
Is chloral hydrate used in the preparation of Mayer's Hematoxylin?Does this mean the solution is controlled as a DEA Schedule IV product?
Yes, chloral hydrate is included in the formulation. No, the solution is not controlled as a DEA Schedule IV product. Once the choral hydrate is mixed with the remaining chemicals, it is impossible to obtain the chloral hydrate in a purified form.
The slides were stained with Mayer's Hematoxylin but the nuclei are not blue. Why?
The nuclei will originally appear as the same color as the solution. Only after the slides have been "blued" will the nuclei appear blue. The pH of the solution is acidic. This acidic pH promotes specific staining. However the slides must be washed in an neutral or alkaline solution for the color to shift to blue. Prolonged warm water washes in tap water are often sufficient for the bluing to occur. To shorten the time spent in washing, various bluing agents are often used to shorten the blueing process. Scott's Tap Water Substitute (Product No. S5134) is one such blueing agent.
There is also a product listed on the website as Hematoxylin Solution According to Mayer. What is the difference between product 51275 and the various Mayer's Hematoxylin solutions?
The basic formulations are the same. Both include chloral hydrate, although some of the concentrations could vary slightly. Product 51275 is not intended for In Vitro Diagnostic Use. Product 51275 is not CE Marked. The various Mayer's Hematoyxlin solutions are intended For In Vitro Diagnostic Use and are also CE Marked. Product inserts are available for various languages.
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Protocols & Articles


Immunohistochemistry Protocol

Immunohistochemical staining is a valuable tool for detecting specific antigens in tissues. In order to perform the standard staining procedure, first the tissue section has to be deparaffinized and ...
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