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MIRRM01 Sigma-Aldrich

MystiCq® microRNA® SYBR® Green qPCR ReadyMix

Low ROX formulation for miRNA RT-qPCR

  •  NACRES NA.55

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Description

General description

The MystiCq microRNA SYBR Green qPCR ReadyMix is one of the integral components of the MystiCq microRNA qPCR Assay System. This ReadyMix is a specially-formulated 2X concentrate with an antibody-based hot-start Taq DNA polymerase and all the optimized components needed for highly specific, reliable, and reproducible microRNA qPCR assays. Simply add the specific MystiCq microRNA qPCR Assay Primer, the MystiCq Universal PCR primer, and cDNA template generated by the MystiCq microRNA cDNA Synthesis Kit and you′re ready to go. Because there are different methods used for fluorescent normalization on different real-time PCR instruments, there is a MystiCq microRNA SYBR Green qPCR ReadyMix available for your platform.

Application

MystiCq microRNA SYBR Green qPCR ReadyMix, Low ROX, is compatible with the following platforms: Applied Biosystems 7500, Applied Biosystems 7500 Fast, Applied Biosystems ViiA 7, Stratagene Mx3000P®, Stratagene Mx3005P®, Stratagene Mx4000.

Features and Benefits

• Exclusively for use with the MystiCq microRNA qPCR Assay Primers, Control Primers, and Universal PCR primer
• 2X concentrated for easy reaction setup with hot-start Taq, dNTPs and optimized buffer
• Passive reference dye (if required) included at the optimal concentration

Packaging

Default reaction volume is 50 μL

100RXN is packaged as 2 X 1.25 mL
500RXN is packaged as 10 X 1.25 mL
2000RXN is packaged as 1 X 50 mL

Legal Information

Mx3000P is a registered trademark of Agilent Technologies, Inc.

Mx3005P is a registered trademark of Agilent Technologies, Inc.

Mx4000 is a trademark of Agilent Technologies, Inc.

MystiCq is a registered trademark of Qiagen Beverly Inc.

ROX is a trademark of Applera Corporation or its subsidiaries in the US and/or certain other countries

ReadyMix is a trademark of Sigma-Aldrich Co. LLC

SYBR is a registered trademark of Life Technologies

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 3
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Protocols & Articles

Protocols

Imprint RIP | uRNA Quantitation | MicroRNA primers | MicroRNA Reverse Transcription

The Imprint® RNA Immunoprecipitation Kit was used to co-purify human argonaute 2 (Ago2)-associated RNAs from HeLa cells, as idicated in the Technical Bulletin. To evaluate the different stringency op...
Carol Kreader
Principal R&D Scientist
Keywords: Buffers, Cell disruption, Immunoprecipitation, Polymerase chain reaction - quantitative, RNA immunoprecipitation, Western blot

MystiCq® MicroRNA® Quantitation System

Technology Overview Workflow Background Protocols miRNA isolation cDNA synthesis miRNA qPCR Advanced information Product selection guides Troubleshooting
Keywords: AGE, Amplification, Buffers, Cell disruption, Centrifugation, Degradations, Diagnostic, Digestions, Diseases, Electrophoresis, Gel electrophoresis, Gene expression, Homogenization, Microarray Analysis, Molecular biology, Nucleic acid annealing, Nucleic acid denaturation, PAGE, Polymerase chain reaction, Polymerase chain reaction - quantitative, Precipitation, Purification, RNA purification, Sample preparations, Separation, Solvents, Titrations

Related Content

PCR Selection Guide

We offer a wide variety of PCR enzymes, master mixes, and PCR protocols to meet your experimental needs for routine PCR, qPCR, or RT-PCR. Our PCR Selection Guide features various filters to sort by, ...
Keywords: Genomics, Polymerase chain reaction, Polymerase chain reaction - quantitative

Peer-Reviewed Papers
15

References

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