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MSRT1 Sigma-Aldrich

MS RT Calibration Mix

Proteomics Retention Time Standard for LC-MS

  •  NACRES NA.24

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Properties

Related Categories Amino Acid, Peptide & Protein Standards, Analytical Standards, Analytical/Chromatography, Mass Spectrometry, Molecular Biology,
Quality Level   200
quality   Proteomics Retention Time Standard for LC-MS
analyte chemical class(es)   amino acids, peptides, proteins
Featured Industry   Food and Beverages
storage temp.   −20°C

Description

General description

MSRT1 is an injection-ready standard, and can serve for quick tests of LC-MS platform performance, comparison of LC gradients and columns, monitoring column and system changes. MSRT1 can be used to predict retention times of peptides across LC-MS platforms. MSRT1 is also applicable to multiple reaction monitoring / selected reaction monitoring (MRM / SRM) analysis.

Components:
Each vial contains various amounts of 14 isotopically labeled synthetic peptides dried via vacuum centrifugation. Each peptide is labeled with either a [13C6,15N2] Lysine (+8 Da), a [13C6,15N4] Arginine (+10 Da), or a [13C6,15N] Leucine (+7 Da).

Application

MS RT Calibration Mix has been used as a reference for iRT (relative retention times) calculations after LC-MS (liquid chromatography–mass spectrometry)/MS analysis. It has been used to check the reliability and specificity of ETD-HCD (electron transfer dissociation-high-energy collisional dissociation) MS3 (mass spectrometry) method for leucine/isoleucine discrimination.

Features and Benefits

General

Complexity
• Defined mixture gives confidence in your instruments analysis

Easily visualized
• Distinct LC-MS peak profile of peptides, readily separated with similar responses
• iRT Table (xls)

Injection-ready
• Decreases prep time

Qualitative:
• Defined mixture of 14 stable isotope labeled (SIL) peptides

Quantitative:
MRM settings provided (Skyline, xls)

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Legal Information

This product is licensed under U.S. Patent No. 7,396,688 and foreign counterparts from E. I. du Pont de Nemours and Company. The purchase of this product conveys to the buyer the nontransferable right to use the purchased amount of the product for research and development only, including services for a third party for consideration. The buyer cannot sell or otherwise transfer this product, its components or materials made using this product or its components to a third party. Information about licenses for excluded uses is available from: E. I. du Pont de Nemours and Company; Attn: Associate Director, Commercial Development; DuPont Experimental Station E268; 200 Powdermill Rd.; Wilmington, DE 19803; 1-877-881-9787 (voice), 1-302-695-1437 (fax), licensing@dupont.com.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 2

Documents

Certificate of Analysis (COA)

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Protocols & Articles

Articles

An LC/MS Peptide Standard for Rapid System Suitability Assessment and Retention Time Translation Across LC/MS Platforms

Standards are critical in liquid chromatography/mass spectrometry (LC/MS) based proteomics to ensure optimal and consistent system performance before, during, and after sample analysis. They may be u...
Kevin Ray, Jim Walters
Reporter US Volume 34.2
Keywords: Chromatography, High performance liquid chromatography, Mass spectrometry, Proteomics

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Peer-Reviewed Papers
15

References

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53561-U Ascentis® Express Peptide ES-C18, 2.7 μm Capillary HPLC Column, 2.7 μm particle size, L × I.D. 15 cm × 1 mm

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