SIGMAFAST™ p-Nitrophenyl phosphate Tablets tablet, To prepare 5 mL

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Properties

Related Categories	p-Nitrophenyl Phosphate Substrate Systems, Alkaline Phosphatase, Alphabetical Listing, Antibodies, Bioassay, Bioassay Substrates, Biochemicals and Reagents, Colorimetric, Detection, Detection Substrates, ELISA Substrates, Enzyme Substrates, Enzymes, Inhibitors, and Substrates, Molecular Biology, Plant Biotechnology, Plant Immunochemistry, Substrates by Enzyme, Substrates for Alkaline Phosphatase, Substrates, Buffers & Blockers, Supplementary Products, Tablets More...
form	tablet
storage temp.	−20°C

Description

Application

Developed for use as a soluble substrate for the detection of alkaline phosphatase activity in Enzyme Immunoassays (EIA). pNPP is the substrate of choice in high sensitivity alkaline phosphatase-based EIA assays.One pNPP tablet and one Tris buffer tablet are dissolved in deionized water and provide a ready-to-use substrate. Each Sigma FAST pNPP tablet set yields a solution containing 1.0 mg/ml pNPP, 0.2 M Tris buffer. EIA applications using pNPP may be read in timed assays or stopped with 3N NaOH for delayed readings. SIGMAFAST^™ require only the addition of distilled or deionized water to prepare an active substrate solution. Tablets are individually packaged in foil packets.

Reconstitution

Each tablet set dissolved in 5 mL deionized water yields a ready-to-use buffered solution containing pNPP.

Legal Information

SIGMAFAST is a trademark of Sigma-Aldrich Co. LLC

Safety & Documentation

Safety Information

RIDADR

NONH for all modes of transport

WGK Germany

3

Documents

Certificate of Analysis

Certificate of Origin

Bulk Quote-Order Product

SDS

Specification Sheet

N1891 - Product Information Sheet (58 KB)

Plant Biotech Guide (201 KB )

Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Colorimetric Alkaline Phosphatase and Peroxidase Substrate Detection Systems

Nitroblue Tetrazolium (NBT) is used with the alkaline phosphatase substrate 5-Bromo- 4-Chloro-3-Indolyl Phosphate (BCIP) in western blotting and immunohistological staining procedures. These substrat...
BioFiles 2008, 3.4, 6.
Keywords: Applications, Enzyme-linked immunosorbent assay, Formulations, Immunohistochemistry, Western blot

References

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