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N3520 Sigma-Aldrich

Nutrient Mixture F-12 Ham

Kaighn′s Modification, with L-glutamine, without sodium bicarbonate, powder, suitable for cell culture

Synonym: Ham’s F-12



Related Categories Cell Culture, Cell Culture Media, Classical Media and Salts, Core Bioreagents, Life Science Reagents for Cell Culture,
sterility   sterile
form   powder
application(s)   cell culture | mammalian: suitable
components   sodium pyruvate: 0.22 g/L
L-glutamine: yes
phenol red: yes
NaHCO3: no
shipped in   ambient
storage temp.   2-8°C


General description

The nutrient mixture Ham′s F-12 is developed by Ham and it has higher levels of amino acids, vitamins and trace elements. Putrescine and linoleic acid are added to the formulation. Nutrient medium was originally designed for the serum-free growth of Chinese hamster ovary and lung cells. When used with whole or dialyzed serum or in combination with hormones and transferrin, it is widely used to grow mammalian and hybridoma cells.


Nutrient Mixture F-12 Ham is used for the following applications:
• HeLa (human cervix adenocarcinoma cells) were grown in this medium (nutrient mixture F-12 Ham) supplemented with 10% heat-inactivated fetal calf serum
• Used in cell culture for different cells during maintenance and incubation (i.e. A549 cell line was maintained as a monolayer in nutrient mixture F-12 HAM Medium along with other components)
• Used in media for the propagation of Cowdria ruminantium
• Used during preparation of primary mixed glial cultures

Kaighn′s modification of Ham′s F-12 and Coon′s F-12 has increased concentrations of amino acids and pyruvate, as well as modified salts (Konigsbergs). This medium was designed to support the growth of differentiated rat and chicken cells, and primary human liver cells.


Formulated to contain 11.1 grams of powder per liter of medium.


Supplement with 2.5 g/L sodium bicarbonate.

Safety & Documentation

Safety Information

GHS07  GHS07
Signal word 
Hazard statements 
NONH for all modes of transport
WGK Germany 
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable
Protocols & Articles


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