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N3520 Sigma-Aldrich

Nutrient Mixture F-12 Ham

Kaighn′s Modification, with L-glutamine, without sodium bicarbonate, powder, suitable for cell culture

Synonym: Ham’s F-12

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Properties

Related Categories Cell Culture, Cell Culture Media, Classical Media and Salts, Core Bioreagents, Life Science Reagents for Cell Culture,
sterility   sterile
form   powder
application(s)   cell culture | mammalian: suitable
components   sodium pyruvate: 0.22 g/L
L-glutamine: yes
phenol red: yes
NaHCO3: no
HEPES: no
shipped in   ambient
storage temp.   2-8°C

Description

General description

The nutrient mixture Ham′s F-12 is developed by Ham and it has higher levels of amino acids, vitamins and trace elements. Putrescine and linoleic acid are added to the formulation. Nutrient medium was originally designed for the serum-free growth of Chinese hamster ovary and lung cells. When used with whole or dialyzed serum or in combination with hormones and transferrin, it is widely used to grow mammalian and hybridoma cells.

Application

Nutrient Mixture F-12 Ham is used for the following applications:
• HeLa (human cervix adenocarcinoma cells) were grown in this medium (nutrient mixture F-12 Ham) supplemented with 10% heat-inactivated fetal calf serum
• Used in cell culture for different cells during maintenance and incubation (i.e. A549 cell line was maintained as a monolayer in nutrient mixture F-12 HAM Medium along with other components)
• Used in media for the propagation of Cowdria ruminantium
• Used during preparation of primary mixed glial cultures

Kaighn′s modification of Ham′s F-12 and Coon′s F-12 has increased concentrations of amino acids and pyruvate, as well as modified salts (Konigsbergs). This medium was designed to support the growth of differentiated rat and chicken cells, and primary human liver cells.

Quantity

Formulated to contain 11.1 grams of powder per liter of medium.

Reconstitution

Supplement with 2.5 g/L sodium bicarbonate.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
2

Single- & multi-channel pipettes from BRAND

Pipette Controller from Brand
Protocols & Articles

Protocols

Cell Culture Protocol 10: Testing Cells for Mycoplasma Contamination by Hoechst DNA Staining

DNA staining methods such as indirect Hoechst staining techniques are quick with results available within 72 hours which compares favorably with 4 weeks for mycoplasma detection by culture isolation....
ECACC Laboratory Handbook 4th Edition
Keywords: Cell culture, Culture media, Detection methods, Indicators, Polymerase chain reaction

Cell Culture Protocol 2: Thawing of Frozen Cell Lines

Many cultures obtained from a culture collection, such as ECACC, will arrive frozen and in order to use the cells they must be thawed and put into culture. It is vital to thaw cells correctly in orde...
ECACC Laboratory Handbook 4th Edition
Keywords: Cell culture, Centrifugation, Culture media

Cell Culture Protocol 3: Subculture of Adherent Cell Lines

Adherent cell lines will grow in vitro until they have covered the surface area available or the medium is depleted of nutrients. At this point the cell lines should be subcultured or passaged in ord...
ECACC Laboratory Handbook 4th Edition
Keywords: Adhesion, Cell culture, Culture media

Cell Culture Protocol 4: Subculture of Semi-Adherent Cell Lines

Some cell lines grow as a mixed population (e.g. B95-8 - marmoset) where a proportion of cells do not attach to the tissue culture flask and remain in suspension. Therefore, to maintain this heteroge...
ECACC Laboratory Handbook 4th Edition
Keywords: Cell culture, Culture media

Cell Culture Protocol 5: Subculture of Suspension Cell Lines

In general terms, cell lines derived from blood (e.g. lymphocytes) grow in suspension cultures. Cells may grow as single cells or in clumps (e.g. EBV transformed lymphoblastic cell lines). For these ...
ECACC Laboratory Handbook 4th Edition
Keywords: Cell culture, Centrifugation, Culture media, Growth factors

Cell Culture Protocol 6: Cell Counting Using a Hemocytometer

For the majority of manipulations using cell lines, such as transfections, cell fusion techniques, cryopreservation and subculture routines it is necessary to quantify the number of cells prior to us...
ECACC Laboratory Handbook 4th Edition
Keywords: Carcinogens, Cell culture, Cryopreservation, Culture media, Flow cytometry

Powdered Media Preparation Instructions

Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture. The entire contents of each package should be used immediately after opening. Preparing m...

Related Content

Cell Culture Troubleshooting Guide

Cell culture has become one of the most fundamental techniques for modeling biological systems, and is of increasing importance in the biotechnology and pharmaceutical sectors as well as an essential...
Keywords: Antibiotics, Apoptosis, Cell attachment, Cell culture, Cryopreservation, Filtration, Growth factors, Pharmaceutical, Phase transitions

Peer-Reviewed Papers
15

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