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N4762 Sigma-Aldrich

Newborn Calf Serum

USA origin, Heat Inactivated, sterile-filtered, suitable for cell culture

Synonym: NBCS, NBS, NCS, calf sera, calf serum, newborn calf sera

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Properties

Related Categories Cell Culture, Fetal Bovine Serum (FBS) and Other Animal Sera, Newborn, Calf and Adult Bovine Sera More...
biological source   bovine (calf)
sterility   sterile-filtered
composition   hemoglobin, ≤20 mg/mL
origin   USA origin
application(s)   cell culture | mammalian: suitable
impurities   ≤100 EU/mL endotoxin
shipped in   dry ice
storage temp.   −20°C

Description

Application

NCS is useful in a wide range of cell culture and organ preservation applications. For example, it is used in cornea preservation and cryopreservation of bovine embryos. NCS is used in cell culture to support the maturation of oocytes; to study the function of tubular epithelial cells; and to develop primary cultures of cells such as visceral adipocytes.

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Preparation Note

Collected from calves that are 10 days old or less.
Prepared by heating at 56°C for 30 minutes.

Analysis Note

Endotoxin tested

Safety & Documentation

Safety Information

Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
How do I get lot-specific information or a Certificate of Analysis?
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
How do I find price and availability?
There are several ways to find pricing and availability for our products.Once you log onto our website, you will find the price and availability displayed on the product detail page.You can contact any of our Customer Sales and Service offices to receive a quote. USA customers: 1-800-325-3010 orview local office numbers.
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
Why is serum Heat-Inactivated (HI)?
Heat inactivation of serum is performed to inactivate complement (which can cause cell lysis or downstream problems in immunoassays). If there are immunoglobulins present in the serum that can bind to the cells in culture (either specifically or non-specifically), there is a chance for antibody-mediated complement cytotoxicity (ADCC, causing cell death). It may also reduce the titer of some mycoplasma and some viruses. Often, heat-inactivated serum is used because of the adoption of a previous protocol. It is mainly used when culturing hematopoetic cells or performing immunological type assays such as mixed-lymphocyte reactions or neutralization assays.FBS may not be heat inactivated as this is not required for all applications. It limits the loss of the other protein activity that may be minimally present in the serum.A protocol for heat inactivation:1. Allow serum containers to come to room temperature. Swirl bottles of serum immediately before adding to the water bath.2. A water-filled control bottles (T1) should be placed in a 56 °C water bath. Place the containers in the water bath up to the serum line. Do not completely submerge the containers. When the temperature of T1 reaches 56 °C, start the timer set for 30 minutes. Do not allow temperatures to exceed 56 °C. Higher temperatures will denature the proteins, gel the serum or alter the performance of the serum.3. Gently swirl the bottles every 5 to 10 minutes while in the water bath and check the temperature in the control bottle. It is not uncommon for plastic containers to bow slightly after heat treatment. This is not harmful to the serum.4. At the end of 30 minutes remove the serum bottles from the water bath. Aliquot the serum into smaller volumes and freeze. Sigma-Aldrich does not recommend repeated thaw-freeze cycles.
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Protocols & Articles

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Peer-Reviewed Papers
15

References

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