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NA0200 Sigma-Aldrich

GenElute HP Plasmid Midiprep Kit

Green Alternative

sufficient for 25 purifications

Synonym: Gen Elute, GenElute HP Plasmid Midiprep Kit

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Description

General description

We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has Inherently Safer Chemistry, compared to the standard use of phenol and chloroform to perform DNA extractions.

Application

GenElute HP Plasmid Midiprep Kit has been used to isolate and purify plasmids.

Features and Benefits

• From harvested bacterial culture to pure plasmid DNA in 30 minutes or less
• Up to 350 μg of high-copy plasmid DNA
• Flexibility of a vacuum or spin format
• No phenol/chloroform extraction or alcohol precipitation required
• Contains fewer plastic components, reducing the amount of waste

Other Notes

For additional information, please see www.sigma-aldrich.com/genelutehp.

Legal Information

GenElute is a trademark of Sigma-Aldrich Co. LLC

Safety & Documentation

Safety Information

Symbol 
Signal word 
Warning
Hazard statements 
Precautionary statements 
Target organs 
Central nervous system
RIDADR 
UN 3316 9

Documents

Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Protocols & Articles

Articles

Creating Transgenic Mice using CRISPR-Cas9 Genome Editing

While several genome editing tools have been developed in recent years, including zinc finger-based strategies and TALENs (transcription activator-like effector nucleases), none have been as efficien...
Keywords: Amplification, Cell culture, Enzyme-linked immunosorbent assay, Gene expression, Genetic, Genetics, Immunohistochemistry, Immunology, Nutrition, Polymerase chain reaction, Recombination, Southern blot, Transcription, Transfection, Western blot

Plasmid DNA Purification Overview

Extraction of macromolecules such as DNA, RNA, and protein is common in molecular biology research. The process of extraction and purification of nucleic acids has evolved from being a complex and la...
Keywords: Centrifugation, Chromatography, Cloning, DNA purification, Digestions, Genetic, Ion Exchange, Molecular biology, Nucleic acid denaturation, Nucleic acid purification, Polymerase chain reaction, Precipitation, Purification, Sequencing, Transcription, transformation

Peer-Reviewed Papers
15

References

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Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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