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NA9604 Sigma-Aldrich

GenElute HP 96-Well Plasmid Miniprep Kit

sufficient for 4 96-well plate purifications

Synonym: Gen Elute, GenElute HP Plasmid Kit, High Throughput Purification, Plasmid Purification

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Properties

Related Categories DNA & RNA Purification, High-Throughput Purification Kits, Molecular Biology, Plasmid DNA Purification
usage   sufficient for 4 96-well plate purifications

Description

Features and Benefits

• Up to 10 μg of high-copy plasmid DNA per well
• Flexibility of both manual and automated formats
• Optimized for production of virus titers from MISSION shRNA plasmid vectors
• No phenol/chloroform extraction or alcohol precipitation required

Application

The purified plasmid DNA is suitable for a wide variety of molecular biology applications, including restriction digestions and sequencing.

General description

An overnight recombinant E. coli culture is harvested with centrifugation and subjected to a modified alkaline-SDS lysis procedure. Bacterial cells are harvested by centrifugation and resuspended in Resuspension Solution containing RNase. The cells are then lysed and neutralized followed by the addition of Binding Solution. The prepared lysate is then transferred to a Sigma HP Filter Plate assembled in a vacuum manifold where it is filtered and collected into a deep well plate. The vacuum manifold is rearranged and the filtered lysate is transferred to the Sigma HP Binding Plate. The plasmid DNA is captured onto the silica based membrane and impurities are removed by successive wash steps. Finally, the binding plate is dried and the plasmid DNA is eluted in Elution Solution.

The GenElute HP 96 Well Plasmid Miniprep Kit offers a simple, rapid, costeffective solution for high-throughput purification of plasmid DNA from recombinant E. coli cultures. By combining silica-binding technology and the convenience of a vacuum format, up to 10 μg of high copy plasmid DNA per well can be recovered from 1.3 mL of culture in less than 50 minutes. Actual yields and the optimum volume of culture depend on the plasmid and culture medium used.

Legal Information

GenElute is a trademark of Sigma-Aldrich Co. LLC

Safety & Documentation

Safety Information

Symbol 
Signal word 
Warning
Hazard statements 
Precautionary statements 
RIDADR 
UN 2924 8(3) / PGIII
Flash Point(F) 
77 °F
Flash Point(C) 
25 °C

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Protocols & Articles

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Automated Protocols for Life Science Research

Our scientists have validated all of the automated methods available below. Automation protocols are also provided as instruction for each of the methods. These protocols supply information such as i...
Keywords: Amplification, Polymerase chain reaction, Polymerase chain reaction - quantitative

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