• USA Home
  • P3201 - Platelet-Derived Growth Factor-BB human

P3201 Sigma-Aldrich

Platelet-Derived Growth Factor-BB human

PDGF-BB, recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture

Synonym: PDGF-BB



Related Categories Angiogenesis, Angiogenic Factors, Cancer Research, Cell Biology, Cell Culture,
Quality Level   200
biological source   human
recombinant   expressed in E. coli
assay   ≥98% (HPLC and SDS-PAGE)
form   lyophilized powder
potency   1.0-3.0 ng/mL EC50
quality   endotoxin tested
mol wt   ~25 kDa
packaging   pkg of 10 μg
  pkg of 50 μg
storage condition   avoid repeated freeze/thaw cycles
application(s)   cell culture | mammalian: suitable
impurities   ≤1 EU/μg
UniProt accession no.   P01127
storage temp.   −20°C
Gene Information   human ... PDGFB(5155)


General description

Platelet-derived growth factor (PDGF) is expressed as homo and heterodimers : PDGF-AB FDGF-AA, PDGF-BB, PDGF-CC and PDGF-DD. The PDGF gene is highly conserved mapped to human chromosome 7p22.3. The active form of Pdgf exists as disulfide-linked dimers. It consists of cystine-knot-fold growth factor domain, which is highly conserved, except for the N-terminal prodomain, which shows variation in length among the isoforms.


Platelet-Derived Growth Factor-BB human has been used :
• As a growth supplement in proximal tubule and glomerular explant cell culture
• In three-dimensional scaffold preparation
• To study the effect of brivanib on growth factor signaling pathways
• In transwell migration assay

Biochem/physiol Actions

Platelet-derived growth factor (PDGF) elicits multifunctional actions with a variety of cells. It is mitogenic to mesoderm-derived cells, such as dermal and tendon fibroblasts, vascular smooth muscle cells, glial cells, and chondrocytes. PDGF significantly activates chemotaxis of neutrophils, monocytes, and fibroblasts. It increases the synthesis of phospholipids, cholesterol esters, glycogen and prostaglandins, and modulates LDL (low density lipoprotein) receptor binding.
PDGF stimulates fibroblast proliferation and extracellular matrix production in order to facilitate tissue repair. PDGF-BB isomer is found to be critical in angiogenesis and is highly expressed in tumor, mediating oncogenic pericyte recruitment. PDGF-BB is an important regulator of bone homeostasis, repair and regeneration.

Other Notes

Dimer of the B chain of human PDGF.

Physical form

Lyophilized from a 0.2 μm filtered solution in 30 mM acetic acid.

Analysis Note

The biological activity is measured by its ability to stimulate 3H-thymidine incorporation in quiescent NR6R-3T3 fibroblasts.

Safety & Documentation

Safety Information

Personal Protective Equipment 
NONH for all modes of transport
WGK Germany 
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable


Certificate of Analysis (COA)

Please Enter a Lot Number

Certificate of Origin (COO)

Please Enter a Lot Number
Protocols & Articles


Cellular Derived Blood Proteins and Enzymes

Cell damage is induced by reactive oxygen species (ROS). ROS are either free radicals, reactive anions containing oxygen atoms, or molecules containing oxygen atoms that can either produce free radic...
BioFiles 2006, 1.5, 4.
Keywords: Aerobic, Alzheimer Disease, Bacterial conjugations, Biofiles, Cancer, Metabolism, Oxidations, Parkinson Disease, Reductions, Respiration

Platelet Derived Growth Factors (PDGF)

Platelet-Derived Growth Factor is the principal mitogen found in mammalian serum and is released from platelets during clot formation.1 PDGF elicits multifunctional actions with a variety of cells, i...
Jennifer Fries
BioFiles 2009, 4.5, 20.
Keywords: Atomic absorption spectroscopy, Growth factors, Purification, Type

Related Content

Cell Culture Troubleshooting Guide

Cell culture has become one of the most fundamental techniques for modeling biological systems, and is of increasing importance in the biotechnology and pharmaceutical sectors as well as an essential...
Keywords: Antibiotics, Apoptosis, Cell attachment, Cell culture, Cryopreservation, Filtration, Growth factors, Pharmaceutical, Phase transitions

Peer-Reviewed Papers


Related Products

related product

Product #


Add to Cart

92210 Timestrip Plus 0 °C
06693 Timestrip Plus -20 °C

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?