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P4333 Sigma-Aldrich

Penicillin-Streptomycin

Solution stabilized, with 10,000 units penicillin and 10 mg streptomycin/mL, sterile-filtered, BioReagent, suitable for cell culture

Synonym: Pen-Strep

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Properties

Related Categories ADME/Tox Products, Antibacterial, Antibiotic Solutions, Antibiotics, Antibiotics A to Z,
InChI Key   KMOOCZWLFBSQCW-XFOLRDBHSA-N
sterility   sterile-filtered
product line   BioReagent
form   liquid
concentration   100 ×
application(s)   cell culture | mammalian: suitable
impurities   endotoxin, tested
suitability   suitable for cell culture
Mode of action   cell wall synthesis | interferes
  protein synthesis | interferes
antibiotic activity spectrum   Gram-negative bacteria
  Gram-positive bacteria
shipped in   dry ice
storage temp.   −20°C

Description

Components

This product is formulated to contain 10,000 units penicillin and 10 mg streptomycin/mL solubilized in a proprietary citrate buffer.

Application

Penicillin-Streptomycin is used to supplement cell culture media to control bacterial contamination. It is recommended for use in cell culture applications at 10 mL/L.
Penicillin streptomycin solution has been used as a supplement in different types of media, including RGC (retinal ganglion cells) culture, α-MEM (minimum essential medium) growth medium, Dulbecco′s modified Eagle′s medium (DMEM)/nutrient mixture F-12 Ham, DMEM and DMEM/F-12 + GlutaMax.

General description

Penicillin Streptomycin solution, also known as Pen Strep, is the most commonly used antibiotic solution for the culture of mammalian cells. Penicillin Streptomycin solution is used to maintain sterile conditions during cell culture, and they show few adverse effects on the cultured cells.

Other Notes

20ml,100ml

Keep container tightly closed in a dry and well-ventilated place. Containers which are opened must be carefully resealed and kept upright to prevent leakage.Storage class (TRGS 510): Non Combustible Liquids

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

Certificate of Origin


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Cell Culture Guide
Protocols & Articles

Articles

3D Organoid Culture: New In Vitro Models of Development and Disease

Poster T-3056: Efficient Differentiation Of Human Pluripotent Stem Cell To Branching Lung Bud Organoids & Maturation Into Alveolar Lung Organoids.
Keywords: Adhesion, Cancer, Cell attachment, Cell culture, Cryopreservation, Diseases, Growth factors, Infectious Diseases, Processes and Functions, Protein assay, Vitamins

Protocols

A transcription activator-like effector toolbox for genome engineering

Neville E Sanjana a, b , Le Cong a, b, c , Yang Zhou a, b , Margaret M Cunniff a, b , Guoping Feng a, b , Feng Zhang a, b
Keywords: Peptide synthesis, Polymerase chain reaction, Transcription

Induced Pluripotent Stem Cell Reprogramming Protocols

Introduction Methods     Reprogramming of Human Fibroblasts using Non-Integrating Self-Replicating RNA Vectors     Reprogramming of Peripheral Blood Mononuclear Cells (PBMCs) using STEMCCA Lentiviral...
Keywords: Apoptosis, Cell culture, Culture media, Gene expression, Growth factors, Immunocytochemistry, Polymerase chain reaction, Respiratory, Transduction, Transfection

Lentiviral Transduction Protocol

Prepare mammalian cells so that they are growing exponentially and are no more than 70-80% confluent before transduction.
Keywords: Cell culture, Gene expression, Molecular biology, Transduction

Organelle isolation: functional mitochondria from mouse liver, muscle and cultured filroblasts

Mitochondria participate in key metabolic reactions of the cell and regulate crucial signaling pathways including apoptosis. Although several approaches are available to study mitochondrial function ...
Keywords: Apoptosis, Buffers, Cell culture, Cell disruption, Centrifugation, Diffusion, Genetic, Genetics, Homogenization, Morphogenesis, Oxidations, Phase transitions, Phosphorylations, Respiration, Respiratory, Solvents

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Antibiotics Selection Guide

The Antibiotic Selection Guide enables researchers to spend more time on their research discoveries and less time seeking established antibiotic information. Link to hundreds of antibiotics for conta...
Keywords: Antibiotics, Cell biology, Cell culture, Genetic, High performance liquid chromatography, Reversed-phase chromatography

Cell Culture Troubleshooting Guide

Cell culture has become one of the most fundamental techniques for modeling biological systems, and is of increasing importance in the biotechnology and pharmaceutical sectors as well as an essential...
Keywords: Antibiotics, Apoptosis, Cell attachment, Cell culture, Cryopreservation, Filtration, Growth factors, Pharmaceutical, Phase transitions

Peer-Reviewed Papers
15

References

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