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  • P4458 - Penicillin-Streptomycin

P4458 Sigma-Aldrich


Solution Stabilized, with 5,000 units penicillin and 5mg streptomycin/mL, sterile-filtered, BioReagent, suitable for cell culture

Synonym: Pen-Strep

  •  NACRES NA.76



Related Categories Antibacterial, Antibiotic Solutions, Antibiotics, Antibiotics A to Z, Antibiotics N-S,
Quality Level   200
sterility   sterile-filtered
product line   BioReagent
form   liquid
concentration   50 ×
application(s)   cell culture | mammalian: suitable
impurities   endotoxin, tested
Mode of action   cell wall synthesis | interferes
  protein synthesis | interferes
antibiotic activity spectrum   Gram-negative bacteria
  Gram-positive bacteria
shipped in   dry ice
storage temp.   −20°C
SMILES string   CC1(C)S[C@@H]2[C@H](NC(=O)Cc3ccccc3)C(=O)N2[C@H]1C(O)=O.CNC[C@H]4[C@H](O)[C@@H](O)[C@H](CCO)O[C@H]4O[C@H]5[C@@H](O[C@@H](C)C5(O)C=O)O[C@H]6[C@H](O)[C@@H](O)[C@H](NC(N)=N)[C@@H](O)[C@@H]6NC(N)=N
InChI   1S/C23H43N7O12.C16H18N2O4S/c1-7-23(38,6-32)18(42-19-8(5-28-2)12(33)13(34)9(40-19)3-4-31)20(39-7)41-17-11(30-22(26)27)14(35)10(29-21(24)25)15(36)16(17)37;1-16(2)12(15(21)22)18-13(20)11(14(18)23-16)17-10(19)8-9-6-4-3-5-7-9/h6-20,28,31,33-38H,3-5H2,1-2H3,(H4,24,25,29)(H4,26,27,30);3-7,11-12,14H,8H2,1-2H3,(H,17,19)(H,21,22)/t7-,8-,9-,10+,11-,12-,13-,14+,15-,16+,17+,18-,19-,20-,23+;11-,12+,14-/m01/s1


General description

Penicillin, a β-lactam antibiotic is extremely active towards bacteria. Penicillins are also termed as penams. It is naturally occurring and has a bicyclic skeleton. Streptomycin is a pseudotrisaccharide. It comprises of a monosubstituted aminocyclitol.


Penicillin-Streptomycin has been used:
• as a supplement in Dulbecco′s modified Eagle′s medium (DMEM) to maintain the Huh7 liver hepatoma cell line
• as a supplement in Roswell park memorial institute-1640 (RPMI) medium to maintain mouse lymphoma YAC-1 cells
• as a component in minimum essential medium Eagle to treat individual mouse spleens for the preparation of splenocytes
• as a supplement in FMX-8 complete medium to culture Chinese hamster ovary cells

Recommended for use in cell culture media at 20 ml/L.

Biochem/physiol Actions

Penicillins can prevent the synthesis of bacterial cell wall. This antibiotic can be used in the treatment of skin and urinary tract infections, upper and lower respiratory infections, and endocarditis. Streptomycin can block Mycobacterium tuberculosis in vitro. Streptomycin inhibits prokaryote protein synthesis by preventing the transition from initiation complex to chain-elongating ribosome, causing miscoding.


This product is formulated to contain 5,000 units penicillin and 5 mg streptomycin/mL solubilized in a proprietary citrate buffer.

Other Notes


Keep container tightly closed in a dry and well-ventilated place. Containers which are opened must be carefully resealed and kept upright to prevent leakage.

Safety & Documentation

Safety Information

NONH for all modes of transport
WGK Germany 
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable


Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
How do I get lot-specific information or a Certificate of Analysis?
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
How do I find price and availability?
There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote. USA customers: 1-800-325-3010 or view local office numbers.
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
What is the method of preparation for Product No. P4458 (Penicillin-Streptomycin); what solution is it made in?
Product No. P4458 is not simply penicillin and streptomycin dissolved in water; it is made in a proprietary buffer.
What is the concentration of the P4458 Penicillin-Streptomycin solution? For example, is it a 50X or 100X?
Product No. P4458 is designed to be added directly to media; you should add 20mL to 1L of medium.There is no intermediate dilution step. So the concentration 50X refers to the 50X dilution into media to the final working concentration.
What is the storage temperature and the stability of the P4458 Penicillin-Streptomycin?
Product No. P4458 (Penicillin-Streptomycin solution) should be stored in the freezer at -20 °C for long-term storage. It may be kept refrigerated at 2-8 °C for up to 40 days.
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