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P4932 Sigma-Aldrich

Polymyxin B sulfate salt

powder, BioReagent, suitable for cell culture

  • CAS Number 1405-20-5

  • Empirical Formula (Hill Notation) C55H96N16O13 · 2H2SO4

  • Molecular Weight 1385.61

  •  EC Number 215-774-7

  •  MDL number MFCD00131991

  •  NACRES NA.76

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Properties

Related Categories Antibacterial, Antibiotics, Antibiotics A to Z, Antibiotics N-S, Antibiotics by Application,
Quality Level   200
product line   BioReagent
form   powder
potency   ≥6,000 USP units per mg
application(s)   cell culture | mammalian: suitable
solubility   H2O: 50 mg/mL
Mode of action   cell membrane | interferes
antibiotic activity spectrum   Gram-negative bacteria
storage temp.   2-8°C

Description

General description

Chemical structure: peptide

Polymyxin B is a member of the polymyxin group of antibiotics, obtained from different strains of Bacillus polymyxa and related species. It contains a cyclic heptapeptide unit and a tripeptide side chain, which is N-acylated by a fatty acyl residue. Polymyxin B sulfate salt is generally used for the treatment of Gram-negative bacterial infections.

Application

Polymyxin B sulfate salt has been used -
• for the treatment of BAC-1.2F5 murine macrophages as an LPS (lipopolysaccharide) inhibitor
• as positive and negative control during microbicidal concentration (MCC) assay

Biochem/physiol Actions

Mode of Action: Polymyxin B Sulfate binds to the lipid A portion of bacterial lipopolysaccharides†, disrupting the cytoplasmic membrane by inducing pores large enough to permit nucleotide leakage in bacterial walls. This disrupts the permeability of the cytoplasmic membrane.

Antimicrobial spectrum: Has bactericidal action on most gram-negative bacilli††, including E. Coli and on most fungi and gram-positive bacteria.

Caution

As supplied, the product should be stored at 2-8°C in the dark. No change was observed in retained samples after three years′ of storage in these conditions. Stock solutions should be sterile filtered and stored at 2-8°C. They are stable at 37°C for 5 days.

Preparation Note

Polymyxin B sulfate is soluble in water at 50 mg/mL, producing a very slightly hazy, colorless to yellow solution. It has only minimal solubility in any organic solvent. For example, it has a solubility of 0.115 mg/mL in ethanol.

Other Notes

1mu,5mu

Keep container tightly closed in a dry and well-ventilated place.Keep in a dry place.

Safety & Documentation

Safety Information

Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
3
RTECS 
TR1150000

Documents

Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Peer-Reviewed Papers
15

References

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