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P7582 Sigma-Aldrich

Amino-terminal FLAG-BAP Fusion Protein



Related Categories FLAG Control Proteins, FLAG System, Molecular Biology, Proteomics, Purification and Detection,
Quality Level   200
form   (Protein is supplied in 10 mM Tris, 120 mM NaCl,
0.05 mM ZnCl2 in 50% glycerol, pH 8.0.)
mol wt   ~49 kDa
shipped in   dry ice
storage temp.   −20°C


General description

Amino-terminal FLAG-BAP Fusion Protein is a 467 amino acid N-terminal FLAG fusion protein of E. coli bacterial alkaline phosphatase (BAP) with a calculated molecular mass of 49.3kDa. The N-terminal FLAG-BAP Fusion Protein migrates as a 45−55kDa band by SDS-PAGE depending on electrophoresis conditions. FLAG-BAP Fusion Protein has biotechnological applications and is used in the screening of recombinant fusion proteins.

The Amino-terminal FLAG-BAP Fusion Protein is a control prorein used to confirm the funtional integrity of the anti-FLAG M1 and M2 monoclonal antibodies in applications such as western blotting, immunoprecipitation, ELISA, electron microscopy, and FACS.


Amino-terminal FLAG-BAP Fusion Protein has been used as a standard in enzyme-linked immunosorbent assay (ELISA) for osteopontin quantification and in western blotting based quantification of Flag-Wnt8 and Drosophila melanogaster transcription factor interactome.

Browse additional application references in our FLAG® Literature portal.

Other Notes

Control protein

Legal Information

FLAG is a registered trademark of Sigma-Aldrich Co. LLC

FLAG-BAP is a trademark of Sigma-Aldrich Co. LLC

Safety & Documentation

Safety Information

NONH for all modes of transport
WGK Germany 
Protocols & Articles


Anti-FLAG® M2 Magnetic Beads

Anti-FLAG® M2 magnetic beads provide an easy, fast and convenient method for the detection and capture of fusion proteins with the FLAG® peptide sequence. Beads are composed of a murine derived, anti...

Fluorescent Multiplex Detection using Antibody Atto Dye Conjugates

Immunoblotting (Western blot transfer) is a common technique in modern proteomics research. After electrophoresis, proteins are immobilized on a nitrocellulose or PVDF membrane and then probed with a...
BioFiles 2011, 6.3, 16.
Keywords: Catalytic combustion detector, Electrophoresis, Immobilization, PAGE, Proteomics, Western blot


Immunoprecipitation of FLAG fusion proteins using monoclonal antibody affinity gels

Immunoprecipitation (IP) can be used for efficient, high-yield isolation and purification of proteins fused to the FLAG® peptide tag. IP is performed with the ANTI-FLAG® M2 affinity gel, which is a h...
Keywords: Cell disruption, Centrifugation, Electrophoresis, Gene expression, Immobilization, Immunoprecipitation, Individual protein Immunoprecipitation, Nucleic acid denaturation, PAGE, Precipitation, Purification, Sample preparations, Size-exclusion chromatography, Tagged proteins

Peer-Reviewed Papers


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92210 Timestrip Plus 0 °C
06693 Timestrip Plus -20 °C

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