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P8203 Sigma-Aldrich

PIPES

BioXtra, ≥99% (titration)

Synonym: 1,4-Piperazinediethanesulfonic acid, Piperazine-1,4-bis(2-ethanesulfonic acid), Piperazine-N,N′-bis(2-ethanesulfonic acid)

  • CAS Number 5625-37-6

  • Empirical Formula (Hill Notation) C8H18N2O6S2

  • Molecular Weight 302.37

  •  Beilstein Registry Number 817713

  •  EC Number 227-057-6

  •  MDL number MFCD00006159

  •  PubChem Substance ID 24898917

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Properties

Related Categories BioXtra Buffers, Biochemicals and Reagents, Biological Buffers, Buffers A to Z, Good’s Buffers More...
InChI Key   IHPYMWDTONKSCO-UHFFFAOYSA-N
Quality Level   ELITE
product line   BioXtra
assay   ≥99% (titration)
impurities   Insoluble matter, passes filter test
ign. residue (900°C)   ≤0.5% (as SO4)
loss   ≤0.5% loss on drying, 110°C
useful pH range   6.1 - 7.5
pKa (25 °C)   6.8
mp   >300 °C (lit.)
solubility   1 M NaOH: 0.5 M at 20 °C, clear, colorless
anion traces   chloride (Cl-): ≤0.2%
cation traces   Al: ≤0.005%
  Ba: ≤0.0005%
  Bi: ≤0.0005%
  Ca: ≤0.005%
  Cd: ≤0.0005%
  Co: ≤0.0005%
  Cr: ≤0.0005%
  Cu: ≤0.0005%
  Fe: ≤0.0005%
  K: ≤0.005%
  Li: ≤0.0005%
  Mg: ≤0.0005%
  Mn: ≤0.0005%
  Mo: ≤0.0005%
  Na: ≤0.1%
  Ni: ≤0.0005%
  Pb: ≤0.0005%
  Sr: ≤0.0005%
  Zn: ≤0.0005%
absorption   A0.5M/260, 1 M NaOH ≤0.1
  A0.5M/280, 1 M NaOH ≤0.1
Featured Industry   Diagnostic Assay Manufacturing

Description

Linkage

Sigma-Aldrich offers BioPerformance Certified cell culture-tested PIPES (Product No. P1851) as well as several different salts for convenience in buffer preparation.

Packaging

50, 250 g in poly bottle

Preparation Note

Buffers can be prepared by adding a solution of base to PIPES free acid to titrate to the appropriate pH, or by mixing equimolar solutions of the monosodium salt and the disodium salt to titrate to the appropriate pH.

Quality

Trace elemental analyses have been performed on the BioXtra PIPES; Certificate of Analysis provides lot-specific results. BioXtra PIPES is for applications which require tight control of elemental content.

Application

Protocols have been reported on the use of PIPES for separation of glyoxylated RNA in agarose gels, nuclease S1 mapping of RNA, and in ribonuclease protection assay protocols. PIPES has been used as a buffer in glutaraldehyde fixation of tissue samples.,
PIPES has been utilized in protein crystallization., The use of PIPES in the reconstitution of dissociated tubulin
α and β subunits after their resolution on immunoadsorbent gels has been described. PIPES has been recommended for use in buffers for the in vitro study of caspases 3, 6, 7, and 8.
A published study demonstrated the usefulness of PIPES as a non-metal ion complexing buffer in such applications as protein assays. PIPES has been used in cell culture for such applications as the engineering of a thermostable mutant membrane protein in Escherichia coli.

General description

PIPES is a member of the ethanesulfonic acid buffer series, first introduced by Good et al., developed to meet certain criteria: midrange pKa, maximum water solubility and minimum solubility in all other solvents, minimal salt effects, minimal change in pKa with temperature, chemically and enzymatically stable, minimal absorption in visible or UV spectral range and easily synthesized. Since its pKa at 37 °C is near physiological pH, PIPES has applications in cell culture work.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

Certificate of Origin


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Protein Stabilizer
Protocols & Articles

Protocols

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Peer-Reviewed Papers
15

References

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