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P8849 Sigma-Aldrich

Protease Inhibitor Cocktail

for use in purification of Histidine-tagged proteins, DMSO solution

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Description

Specificity

Inhibits serine, cysteine, aspartic, and thermolysin-like proteases, and aminopeptidases.

Packaging

1 mL in poly bottle

5 mL in glass bottle

Application

Optimized and tested for isolation of histidine-tagged proteins. Chelating agents have been omitted to ensure full compatibility with IMAC applications.

Components

AEBSF
Bestatin
E-64
Pepstatin A
Phosphoramidon

Quantity

One mL is recommended for the inhibition of proteases extracted from 20 g of Escherichia coli or 10 g of baculovirus-infected Spodoptera frugiperda pupal ovary cells in a total volume of 100 ml.

This protease inhibitor cocktail has been optimized and tested for histidine-tagged proteins

Preparation Note

This product is supplied as a clear solution in DMSO. One mL of solution is recommended for inhibition of protease activity in 100 mL of cell lysate from 20 g of E. coli cells or 10 g of baculovirus-infected cells.

Safety & Documentation

Safety Information

WGK Germany 
1
Flash Point(F) 
185 °F
Flash Point(C) 
85 °C

Documents

Certificate of Analysis (COA)

Please Enter a Lot Number

Certificate of Origin (COO)

Please Enter a Lot Number

Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
How do I get lot-specific information or a Certificate of Analysis?
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
How do I find price and availability?
There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote. USA customers: 1-800-325-3010 or view local office numbers.
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
What is the difference between Protease Inhibitors P8849 and S8830?
P8849 is a DMSO solution and S8830 is a water soluble tablet. P8849 formulation is considered proprietary but does contain AEBSF, Pepstatin A, Bestatin, E-64, and Phosphoramidon. The formulation of S8830 also contains Aprotinin and Pepstatin A and the exact formulation is given on the product information sheet (under Documents, above).
Can Product No. P8849, Protease Inhibitor Cocktail, be used with with HIS-Select products or any other immobilized metal affinity chromatography (IMAC) products?
This product does not contain EDTA and can be used with sample processing by immobilized metal affinity chromatography (IMAC) suchas used for the purification of histidine-tagged proteins..
How much of Product No. P8849, Protease Inhibitor Cocktail, should be added to the cell lysate?
A 1:100 dilution is the recommended concentration.
My question is not addressed here, how can I contact Technical Service for assistance?
Ask a Scientist here.
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Single- & multi-channel pipettes from BRAND
Protocols & Articles

Articles

Protease Inhibitor Cocktails References

Our portfolio of protease inhibitor cocktails are frequently cited in peer-reviewed scientific literature, suggesting their superior quality and excellent track record. This page lists a sampling of ...
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Peer-Reviewed Papers
15

References

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