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PP0200 Sigma-Aldrich

GlycoProfile I, In-Gel Deglycosylation Kit

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Properties

Related Categories Deglycosylation Kits, Deglycosylation Strategies, Enzymatic Deglycosylation, Glycobiology, Molecular Biology,
Quality Level   200
shelf life   ≥1 yr at 2‑8 °C
storage temp.   2-8°C

Description

Application

The GlycoProfile Enzymatic In-gel N-Deglycosylation Kit is optimized to provide a convenient, reproducible method to N-deglycosylate and digest protein samples from one dimensional (1D) and two dimensional (2D) polyacrylamide gel pieces for subsequent mass spectrometry or HPLC analysis.
This procedure is suitable for Coomassie® Brilliant Blue and colloidal Coomassie stained gels. Silver stained gels may also be used if properly destained.

Principle

The kit includes Proteomics Grade PNGase F and trypsin enzymes necessary for N-linked deglycosylation and tryptic digestion, respectively. The samples can then be desalted and concentrated for analysis by MALDI-TOF MS or electrospray MS with subsequent database searching.

Quantity

Each kit contains reagents sufficient to deglycosylate and digest up to 10 samples.

Legal Information

GlycoProfile is a trademark of Sigma-Aldrich Co. LLC

Kit component also available separately

Description

Product #

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PNGase F from Elizabethkingia meningoseptica, BioReagent, ≥95% (SDS-PAGE), for proteomics 50 U SDS P7367
Trypsin from porcine pancreas, BioReagent, Proteomics Grade, Dimethylated 20 μg SDS T6567
Trypsin Solubilization Reagent    
Invertase Glycoprotein Standard, BioReagent, from Saccharomyces cerevisiae, for proteomics SDS I0408
Acetonitrile, ≥99.93%, BioSyn® 50 mL    
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 3
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable
Protocols & Articles

Articles

Deglycosylation Kits

GlycoProfile™ I Enzymatic In-gel N-Deglycosylation Kit GlycoProfile™ II Enzymatic In-solution N-Deglycosylation Kit Enzymatic Protein Deglycosylation Kit Native Protein Deglycosylation Kit
Glycobiology Analysis Manual, 2nd Edition
Keywords: Degradations, Digestions, Glycosylations, High performance liquid chromatography, Mass spectrometry, PAGE, Proteomics

Glycoprotein Deglycosylation

Aparagine-linked (N-linked) and serine/threonine-linked (O-linked) oligosaccharides are major structural components of many eukaryotic proteins. They perform critical biological functions in protein ...
BioFiles 2007, 2.1, 7.
Keywords: Degradations, Dialysis, Digestions, Filtration, Glycosylations, High performance liquid chromatography, Inflammation, Ligands, Mass spectrometry, Methods, PAGE, Proteomics, Substitutions, Type

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