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R0278 Sigma-Aldrich

RIPA Buffer

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Properties

Related Categories Cell Lysis, Cell Lysis and Protein Extraction, Core Bioreagents, Life Science Reagents for Protein Expression and Purification, Mammalian Cell Lysis,
Quality Level   200
form   liquid
feature   protease free
packaging   pkg of 50 mL
  pkg of 500 mL
application(s)   immunoprecipitation (IP): suitable
color   colorless
pH   7.8-8.2 (25 °C, 1 ×)
storage temp.   2-8°C

Description

General description

RIPA (Radio-Immunoprecipitation Assay) Buffer is supplied as a ready to use solution that requires no preparation. It minimizes non-specific protein-binding interactions to keep background low, while allowing most specific interactions to occur, enabling studies of relevant protein-protein interactions. It is widely used in applications such as immunoprecipitation since most antibodies and protein antigens are not adversely affected by the components of this buffer.

Radioimmunoprecipitation assay buffer (RIPA buffer) is a lysis buffer used for rapid, efficient cell lysis and solubilization of proteins from both adherent and suspension cultured mammalian cells.

Application

RIPA Buffer has been used as a reagent for cell lysis in the following experimental studies:
• Fibroblast-based deep phenotyping of the peripheral tissue, which facilitates the mechanistic disease stratification in sporadic Parkinson′s disease (sPD).
• Demonstrating the inhibition of oxidative phosphorylation (OXPHOS), which can activate the mixed lineage kinase domain-like protein (MLKL)-dependent necroptosis in human lung epithelial cells.

RIPA Buffer enables efficient cell lysis and protein solubilization while avoiding protein degradation and interference with the proteins′ immunoreactivity and biological activity. RIPA Buffer also results in low background in immunoprecipitation and molecular pull-down assays. Compatible with EZview Affinity Gels.

Features and Benefits

1 mL of the RIPA Buffer is used to lyse cells from one 100 mm culture dish (0.5 to 5 * 107 cells) of most adherent mammalian cell lines.

Minimizes non-specific protein binding interactions to ensure low background.

Other Notes

Ready-to-use solution containing 150 mM NaCl, 1.0% IGEPAL® CA-630, 0.5% sodium deoxycholate, 0.1% SDS, 50 mM Tris, pH 8.0.

Legal Information

EZview is a trademark of Sigma-Aldrich Co. LLC

IGEPAL is a registered trademark of Rhodia Operations

Safety & Documentation

Safety Information

Symbol 
Signal word 
Warning
Hazard statements 
RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 3
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
How do I get lot-specific information or a Certificate of Analysis?
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
How do I find price and availability?
There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote. USA customers: 1-800-325-3010 or view local office numbers.
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
After preparing a lysate using Product R0278, RIPA Buffer, how should it be stored? And for how long can the lysate be stored?
Lysates containing the appropriate inhibitor(s) can be stored at -70°C for up to a year.
Can Product R0278, RIPA buffer, be used on tissue samples, or only on cultured cells?
R0278 can be used on tissue, if it is well homogenized.
Does Product R0278, RIPA buffer, contain any protease inhibitors or phosphatase inhibitors?
This buffer does not contain any protease or phosphatase inhibitors, however, they may be added to insure against possible proteolysis.
How does Product R0278, RIPA buffer, compare to Product No. C2978 (CelLytic M)?
RIPA buffer contains SDS, which is considered to be a harsh detergent; it can denature many proteins. C2978 is a buffered solution (pH 7.6) that contains a mild detergent; it is non-denaturing for most proteins.The exact formulation of C2978, however, is proprietary.
I left the bottle ofProduct R0278, RIPA buffer, on my bench for about a week. Can I still use it?
Yes, you should be able to use this product.Although we recommend storage in the refrigerator at 2-8°C for long-term storage, Product No. R0278 may be kept at ambient temperature (our normal shipping condition) for a short time (approximately 2 weeks).
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Protocols & Articles

Articles

A functional assay for microRNA target identification and validation

MicroRNAs (miRNA) are a class of small RNA molecules that regulate numerous critical cellular processes and bind to partially complementary sequences resulting in down-regulation of their target gene...
Joop Gäken, Azim M. Mohamedali, Jie Jiang, Farooq Malik, Doris Stangl, Alexander E. Smith, Constantinos Chronis, Austin G. Kulasekararaj, Nicolas S. B. Thomas, Farzin farzaneh, Mahavash Tavassoli, and Ghulam J. Maufi
Nucleic Acids Research 2012, 1-9
Keywords: Cellular processes, Sequences, Western blot

Protocols

Protein Extraction and Cell Lysis Protocol

All the steps for protein extraction from cells or tissue (fresh or frozen) must be carried out at 2-8 °C. The following is the composition of one common lysis buffer that is used to prepare protein ...
Keywords: BCA assay, Cell disruption, Electrophoresis, Gel electrophoresis, Homogenization, Protein assay, Protein extraction

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Peer-Reviewed Papers
15

References

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