EMAIL THIS PAGE TO A FRIEND
R5158 Sigma-Aldrich

RPMI-1640 Medium

HEPES Modification, With 25 mM HEPES and L-glutamine, liquid, sterile-filtered, suitable for cell culture

Purchase

Properties

Related Categories Cell Culture, Cell Culture Media, Classical Media and Salts, Core Bioreagents, Life Science Reagents for Cell Culture,
sterility   sterile-filtered
form   liquid
application(s)   cell culture | mammalian: suitable
impurities   Endotoxin, tested
pH range   >7.2
components   NaHCO3: 2 g/L
phenol red: 0.0053 g/L
L-glutamine: 0.3 g/L
glucose: 2 g/L (Dextro)
HEPES: 5.96 g/L
shipped in   ambient
storage temp.   2-8°C

Description

General description

This RPMI-1640 medium is supplemented with HEPES. HEPES is a Good’s physiological buffer that helps provide a more stable medium pH than bicarbonate buffers.

Application

RPMI 1640 Medium was developed at Roswell Park Memorial Institute in 1966 by Moore and his co-workers. A modification of McCoy′s 5A Medium, it was formulated to support lymphoblastoid cells in suspension culture, but it has since been shown to support a wide variety of cells that are anchorage dependent. Originally intended to be used with a serum supplement, RPMI 1640 has been shown to support several cell lines in the absence of serum. It has also been widely used in fusion protocols and in the growth of hybrid cells.

Linkage

Need FBS? We can assist with your serum planning. Please visit the Serum Planner for more information.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis (COA)

Please Enter a Lot Number

Single- & multi-channel pipettes from BRAND

Pipette Controller from Brand
Protocols & Articles

Related Content

Cell Culture Troubleshooting Guide

Cell culture has become one of the most fundamental techniques for modeling biological systems, and is of increasing importance in the biotechnology and pharmaceutical sectors as well as an essential...
Keywords: Antibiotics, Apoptosis, Cell attachment, Cell culture, Cryopreservation, Filtration, Growth factors, Pharmaceutical, Phase transitions

Related Products

related product

Product #

Description

Add to Cart

08168 Timestrip Plus 8 °C

supplement

Product #

Description

Add to Cart

F2442 Fetal Bovine Serum, USA origin, sterile-filtered, suitable for cell culture, suitable for hybridoma

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?