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R6504 Sigma-Aldrich

RPMI-1640 Medium

With L-glutamine, without sodium bicarbonate, powder, suitable for cell culture

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Properties

Related Categories Cell Culture, Cell Culture Media, Classical Media and Salts, Core Bioreagents, Life Science Reagents for Cell Culture,
Quality Level   200
sterility   sterile
form   powder
application(s)   cell culture | mammalian: suitable
components   L-glutamine: yes
HEPES: no
phenol red: yes
NaHCO3: no
sodium pyruvate: no
shipped in   ambient
storage temp.   2-8°C

Description

General description

Roswell Park Memorial Institute medium is commonly known as RPMI. It is a form of medium used in cell culture and tissue culture. This medium contains phosphate and is formulated for use in a 5% carbon dioxide atmosphere. It is used for the growth of human lymphoid cells.

Application

RPMI-1640 Medium is used for the following applications:
• used in cell culture medium (i.e. Murine thymoma EL-4 cells were cultured in RPMI 1640 medium supplemented with other components)
• used for the mycobacterial infection of splenocytes (in antibiotic-free RPMI 1640 medium)
• used for cell isolation and culture
• used in culture medium (as one of the component) during lymphocyte separation and culture, ELISPOT Assay, Ex Vivo proliferation assay
• used in medium for gp39 gene expression and CD40-Immunoglobuin binding assays
• used for the preparation of antifungal agents

RPMI 1640 Medium was developed at Roswell Park Memorial Institute in 1966 by Moore and his co-workers. A modification of McCoy′s 5A Medium, it was formulated to support lymphoblastoid cells in suspension culture, but it has since been shown to support a wide variety of cells that are anchorage dependent. Originally intended to be used with a serum supplement, RPMI 1640 has been shown to support several cell lines in the absence of serum. It has also been widely used in fusion protocols and in the growth of hybrid cells.

Quantity

Formulated to contain 10.4 grams of powder per liter of medium.

Reconstitution

Supplement with 2.0 g/L sodium bicarbonate.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
1

Single- & multi-channel pipettes from BRAND

Pipette Controller from Brand
Protocols & Articles

Articles

Elispot assays: State-of-the-art tools for functional analysis of cellular immunology

Under optimal conditions, the enzyme-linked immunosorbent spot (Elispot) assay enables visualization of multiple secretory products from a single responding cell. Thus, the Elispot provides both qual...
Keywords: Absorption, Adsorption, Amplification, Apoptosis, Cancer, Clinical, Cryopreservation, Degradations, Deposition, Detergents, Diagnostic, Diffusion, Diseases, Enzyme activity, Enzyme-linked immunosorbent assay, Evaporation, Filtration, Flow cytometry, Gene expression, Immobilization, Indicators, Microscopy, PAGE, Phase transitions, Polymerase chain reaction, Polymerase chain reaction - quantitative, Precipitation, Reductions, Titrations, Western blot

Protocols

Cell Culture Protocol 10: Testing Cells for Mycoplasma Contamination by Hoechst DNA Staining

DNA staining methods such as indirect Hoechst staining techniques are quick with results available within 72 hours which compares favorably with 4 weeks for mycoplasma detection by culture isolation....
ECACC Laboratory Handbook 4th Edition
Keywords: Cell culture, Culture media, Detection methods, Indicators, Polymerase chain reaction

Cell Culture Protocol 2: Thawing of Frozen Cell Lines

Many cultures obtained from a culture collection, such as ECACC, will arrive frozen and in order to use the cells they must be thawed and put into culture. It is vital to thaw cells correctly in orde...
ECACC Laboratory Handbook 4th Edition
Keywords: Cell culture, Centrifugation, Culture media

Cell Culture Protocol 3: Subculture of Adherent Cell Lines

Adherent cell lines will grow in vitro until they have covered the surface area available or the medium is depleted of nutrients. At this point the cell lines should be subcultured or passaged in ord...
ECACC Laboratory Handbook 4th Edition
Keywords: Adhesion, Cell culture, Culture media

Cell Culture Protocol 4: Subculture of Semi-Adherent Cell Lines

Some cell lines grow as a mixed population (e.g. B95-8 - marmoset) where a proportion of cells do not attach to the tissue culture flask and remain in suspension. Therefore, to maintain this heteroge...
ECACC Laboratory Handbook 4th Edition
Keywords: Cell culture, Culture media

Cell Culture Protocol 5: Subculture of Suspension Cell Lines

In general terms, cell lines derived from blood (e.g. lymphocytes) grow in suspension cultures. Cells may grow as single cells or in clumps (e.g. EBV transformed lymphoblastic cell lines). For these ...
ECACC Laboratory Handbook 4th Edition
Keywords: Cell culture, Centrifugation, Culture media, Growth factors

Cell Culture Protocol 6: Cell Counting Using a Hemocytometer

For the majority of manipulations using cell lines, such as transfections, cell fusion techniques, cryopreservation and subculture routines it is necessary to quantify the number of cells prior to us...
ECACC Laboratory Handbook 4th Edition
Keywords: Carcinogens, Cell culture, Cryopreservation, Culture media, Flow cytometry

Powdered Media Preparation Instructions

Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture. The entire contents of each package should be used immediately after opening. Preparing m...

Related Content

Cell Culture Troubleshooting Guide

Cell culture has become one of the most fundamental techniques for modeling biological systems, and is of increasing importance in the biotechnology and pharmaceutical sectors as well as an essential...
Keywords: Antibiotics, Apoptosis, Cell attachment, Cell culture, Cryopreservation, Filtration, Growth factors, Pharmaceutical, Phase transitions

RPMI Media - RPMI-1640 Media

RPMI-1640 was developed by Moore et. al. at Roswell Park Memorial Institute, hence the acronym RPMI. The formulation is based on the RPMI-1630 series of media utilizing a bicarbonate buffering system...
Keywords: Vitamins

Peer-Reviewed Papers
15

References

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