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RNB100 Sigma-Aldrich

GenElute Total RNA Purification Kit

Green Alternative



Related Categories 12 Principles Aligned Products, Chemical Synthesis, DNA & RNA Purification, Greener Alternative Products, Greener Life Science Products,
feature   DNA free (removal without the use of enzyme)
storage condition   room temperature
greener alternative product characteristics   Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.
application(s)   RT-PCR: suitable
shipped in   ambient
storage temp.   room temp


General description

Sigma’s GenElute Total RNA Purification Kit provides a rapid method for the isolation and purification of total RNA from cultured animal cells, tissue samples, blood, plasma, serum, bacteria, yeast, fungi, plants and viruses. The kit purifies all sizes of RNA, from large mRNA and ribosomal RNA down to microRNA (miRNA) and small interfering RNA (siRNA). The RNA is preferentially purified from other cellular components such as proteins, without the use of phenol or chloroform. The purified RNA is of the highest integrity, and can be used in a number of downstream applications including real time PCR, reverse transcription PCR, Northern blotting, RNase protection and primer extension, and expression array assays.

• Isolate true total RNA including siRNA and microRNA
• Rapidly remove contaminating genomic DNA without the use of enzymes
• No phenol or chloroform extractions
• Purify high-quality RNA in 20 minutes
• Extract RNA from as little as a single cell
• Isolate from a wide variety of specimens


GenElute Total RNA Purification Kit has been used to:
• Quantitative, real-time RT-PCR for both large mRNA and small RNA including miRNA
• RT-PCR for both large mRNA and small RNA including miRNA
• Expression profiling
• Next Generation Sequencing for RNA and miRNA
• miRNA from plasma for discovery
• microRNA cloning and amplification
• PCR-based virus detection
• PCR-based viable bacteria detection
• Northern blotting
• RNase protection
• Primer extension
• miRNA array
• RNA-Seq
• Nanostring
• Fluidigm
• Droplet/Digital PCR
• Isolate total RNA of rat kidney tissues

GenElute Total RNA Purification Kit is used for the extraction of total RNA:
• From whole blood samples in the detection of Hepatitis C Virus using a reverse transcriptase-polymerase spiral reaction (RT-PSR) system.
• From an overnight culture of bacterial strains in TSB medium in determining the effects of curcumin and silymarin on virulence and carbapenemase genes among multidrug-resistant Escherichia coli clinical isolates.

Features and Benefits

• No phenol: chloroform extractions - Total RNA is isolated without the use of harmful chemicals such as phenol or chloroform
• Isolate total RNA from very small samples - Total RNA has been isolated and detected from as little as a single animal cell
• Extremely efficient isolation of low abundance microRNA - GenElute′s Total RNA Purification Kit has been shown to be extremely efficient at recovering low abundance microRNA from plasma samples
• Isolate a diversity of RNA species - All RNA species can be isolated, from large mRNA and ribosomal RNA down to microRNA (miRNA) and small interfering RNA (siRNA)
• Fast and easy processing - Rapid spin-column format allows for the processing of 10 samples in 20 minutes
• Isolate total RNA from a broad input source - Total RNA has been isolated from cultured animal cells, small tissue samples, LCM samples, bacteria, yeast, fungi, plants, viruses and various bodily fluid including blood, plasma, serum, saliva, nasal or throat swabs
• No need for carrier RNA - Isolate all sizes of RNA without the use of carrier RNA
• Multiple kit sizes available - This kit is available in both 50 prep and 100 prep sizes

Unit Definition


Other Notes

We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has Inherently Safer Chemistry, compared to the standard use of phenol and chloroform to perform DNA extractions.

Legal Information

GenElute is a trademark of Sigma-Aldrich Co. LLC

Safety & Documentation

Safety Information

Signal word 
Hazard statements 
Supplemental Hazard Statements 
UN 3265 8 / PGIII


Certificate of Analysis (COA)

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Protocols & Articles


Viral RNA Purification Protocol Using GenElute™ Spin Prep Kits

Research use only. Not for use in diagnostic procedures. This protocol is relevant for catalog number RNB100.
Keywords: Anticoagulants, Cell culture, Cell disruption, Chromatography, Coagulants, Column chromatography, Diagnostic, Gene expression, Microarray Analysis, Phase transitions, Polymerase chain reaction, Purification, RNA purification, Separation, Sequencing

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