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S0192 Sigma-Aldrich

Stemline® II Hematopoietic Stem Cell Expansion Medium

Serum-free, contains L-glutamine, liquid, sterile-filtered, suitable for cell culture

  •  NACRES NA.81

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Properties

Related Categories 3D Stem Cell Culture using 3D Biotek 3D Insert Scaffolds, Cell Biology, Cell Culture, Cell Culture Supplies, Culture,
Quality Level   500
sterility   sterile-filtered
form   liquid
storage condition   protect from light
application(s)   cell culture | mammalian: suitable
shipped in   wet ice
storage temp.   2-8°C

Description

General description

The second generation of Sigma′s hematopoietic stem cell expansion media family, Stemline II has been developed to optimize the balance of differientiated and undifferentiated cells while maximizing their expansion. Compatible with Hematopoietic Stem Cells (HSC) from bone marrow, cord blood, and mobilized peripheral blood, Stemline II has been shown to lead to significant increases in cell expansion from all three sources. Through flow cytometric analysis of clinical-scale expansions, Stemline II has also demonstrated a higher capacity than other commercially available media for the expansion of CD34+/CD38+ late progenitors required for short-term engraftment. Human cord blood cells expanded in Stemline Media demonstrate impressive self-revewal when transplanted into immunodeficient NOC/SCID mice, illustrating Stemline′s utility in true functional trial.

Stemline Hematopoietic Stem Cell Expansion Medium free of serum and all other animal-derived components with the exception of human serum albumin. This exclusion increases performance consistency and elimates safety risks associated with potential adventitious agents.

Produced in a GMP state-of-the-art facility, Stemline Hematopoietic Stem Cell Expansion Medium is clearly an excellent choice for your HSC applications.

Please click here to contact us if you are considering including the use of this product in an Investigational New Drug application.

Reconstitution

Must be supplemented with growth factors and cytokines according to individual user protocols.

Legal Information

Stemline is a registered trademark of Sigma-Aldrich Co. LLC

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
1

Pipette Controller from Brand
Protocols & Articles

Protocols

Hematopoietic Stem Cell Culture Protocols

Isolation of Human CD34+ Hematopoietic Progenitor Cells Expansion of Human CD34+ Hematopoietic Progenitor Cells The Colony Formation Unit (CFU) Cell Assay  
Keywords: Cell culture, Centrifugation, Clinical, Culture media, Flow cytometry, Gene expression

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Peer-Reviewed Papers
15

References

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Description

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Z701475 Stem Cells Handbook, S. Sell, ed., Humana Press, 2003, 528 pp., hard cover

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