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S0196 Sigma-Aldrich

Stemline® Keratinocyte Medium II

Serum-free, Without L-glutamine, calcium-free, with HEPES, Sodium Bicarbonate, and phenol red, liquid, sterile-filtered

Synonym: Extended Life Span keratinocyte medium



Related Categories 3D Stem Cell Culture using 3D Biotek 3D Insert Scaffolds, Cell Biology, Cell Culture, Cell Culture Supplies, Culture,
Quality Level   200
description   Requires supplementation: S9945, G8541, G7513, G6392, C7902
sterility   sterile-filtered
form   liquid
quality   Serum-free
  Without L-glutamine, calcium-free, with HEPES, Sodium Bicarbonate, and phenol red
storage temp.   2-8°C


General description

Developed to promote the optimal expansion of human epidermal keratinocytes from adult and neonatal sources, Stemline Keratinocyte Medium II performs most effectively when supplemented with either Stemline Keratinocyte Growth Supplement (S9945) or Keratinocyte Medium Supplement, BPE-free (K3136).

Stemline Keratinocyte Medium II is free of serum and all other animal components; this exclusion increases performance consistency and elimates safety risks associated with potential adventitious agents.

Produced in a GMP state-of-the-art facility, Stemline Keratinocyte Stem Cell Expansion Medium is clearly an excellent choice for your Keratinocyte applications.


Stemline® Keratinocyte Medium II has been used to culture explants for keratocytes.

Legal Information

Stemgent is a registered trademark of Stemgent, Inc.

Stemline is a registered trademark of Sigma-Aldrich Co. LLC

Safety & Documentation

Safety Information

NONH for all modes of transport
WGK Germany 
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable


Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Protocols & Articles

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