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S4942 Sigma

SYPRO® Ruby Protein Gel Stain

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Description

Caution

Protect from light.

Application

SYPRO ruby protein gel stain has been used for staining of the proteins after sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE).

General description

SYPRO Ruby protein gel stain is a ready-to-use, ultrasensitive, luminescent stain for the detection of proteins separated by polyacrylamide gel electrophoresis (PAGE). This stain, designed especially for use in 2-D PAGE, has proven to be an excellent choice for 1-D PAGE and isoelectric focusing (IEF) gels as well. SYPRO Ruby protein gel stain attains sensitivity comparable to many silver stain techniques. However, unlike silver staining, the SYPRO Ruby gel stain:
• uses a simple staining protocol, with no possibility of overstaining
• delivers a linear quantitation range of over three orders of magnitude
• shows less protein-to-protein variability
• stains glycoproteins, lipoproteins, calcium-binding proteins, fibrillar proteins, and other difficult-to-stain proteins
• will not stain extraneous nucleic acids
• does not interfere with subsequent analysis of proteins by Edman-based sequencing or mass spectrometry

Legal Information

SYPRO is a registered trademark of Life Technologies

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

Certificate of Origin


Same Products - New Packaging
Protocols & Articles

Articles

Electrophoresis - Protein Staining Reagents

To meet the great diversity of protein analysis needs, we offer a wide selection of protein visualization (staining) reagents. EZBlue™ and ProteoSilver™, designed specifically for proteomics, also pe...
Keywords: PAGE, Proteomics

Introduction to PAGE

Polyacrylamide gels are formed by the reaction of acrylamide and bis-acrylamide (N,N’-methylenebisacrylamide) that results in highly cross-linked gel matrix. The gel acts as a sieve through which the...
Keywords: Buffers, Cell disruption, Electrophoresis, Gel electrophoresis, PAGE, Protein electrophoresis, Sample preparations, Separation, Western blot

Protocols

State-of-the-art two-dimensional gel electrophoresis: a key tool of proteomics research

Odile Carrette a , Pierre R Burkhard a, b , Jean-Charles Sanchez a , Denis F Hochstrasser a, c, d
Keywords: 2D Electrophoresis, Absorption, Alkylations, Carcinogens, Clinical, Clinical Chemistry, Deposition, Detergents, Electrophoresis, Gel electrophoresis, High performance liquid chromatography, Immobilization, Isoelectric focusing, Mutagens, Neurology, PAGE, Polymerization reactions, Precipitation, Protein assay, Protein electrophoresis, Proteomics, Reductions, Respiratory, Sample preparations, Separation, Size-exclusion chromatography, Teratogens

Peer-Reviewed Papers
15

References

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S5817 SYPRO® Red Protein Gel Stain

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