• USA Home
  • S8695 - Anti-Supervillin antibody produced in rabbit

S8695 Sigma-Aldrich

Anti-Supervillin antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

Synonym: Anti-Supervillin Isoform 1



Related Categories Alphabetical Index, Antibodies, Primary Antibodies, SU-SY
conjugate   unconjugated
clone   polyclonal
biological source   rabbit
application(s)   indirect immunofluorescence: 10-20 μg/mL using human HeLa cells
  western blot: 2.5-5 μg/mL using whole extracts of differentiated mouse C2 cells
  western blot: 5-10 μg/mL using whole extracts of human HeLa cells
species reactivity   human, mouse
mol wt   antigen ~250 kDa
form   buffered aqueous solution
shipped in   dry ice
storage temp.   −20°C
antibody form   affinity isolated antibody
antibody product type   primary antibodies
UniProt accession no.   O95425
Gene Information   human ... SVIL(6840)
mouse ... Svil(225115)


General description

Supervillin is an F-actin-binding protein originally isolated from bovine neutrophils. It has two isoforms of reported 205/250 kDa. The longer isoform named archvillin is muscle specific. Supervillin is a tightly bound peripheral membrane protein that is concentrated at sites of epithelial cell-cell adhesion. The COOH-terminus of supervillin is homologous to villin/gelsolin. The NH2-terminus contains functional nuclear localization sequences and F-actin and myosin II binding domains. Supervillin is found in many cells of several species, but is most abundant in muscle, bone marrow, thyroid gland and salivary gland.


synthetic peptide corresponding to amino acid residues 900-918 of human supervillin with C-terminal added cysteine, conjugated to KLH. The corresponding sequence differs by two amino acids in mouse.


Anti-Supervillin antibody produced in rabbit has been used in:
• immunoblotting
• chromatin immunoprecipitation (ChIP)
• immunofluorescence

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.


Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Biochem/physiol Actions

Supervillin participates in cell-cell adhesion, motility regulation and information transfer between cell compartments. It has been suggested that supervillin may mediate actin and myosin II filament organization at cholesterol-rich membrane domains. Supervillin has been identified as a transcriptional activator of the androgen receptor. Increased levels of supervillin are found in many carcinoma cell lines, including HeLa S3 cervical carcinoma, SW480 adenocarcinoma and A549 lung carcinoma cells.

Safety & Documentation

Safety Information

NONH for all modes of transport
WGK Germany 
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable


Certificate of Analysis (COA)

Please Enter a Lot Number
Protocols & Articles


Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy


Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

Related Content

Antibody Explorer | Buy Primary Antibodies & Secondary Antibodies

Primary, Secondary and Recombinant Monoclonal Antibodies Providing highly cited primary and secondary antibodies, we have you covered for your ELISA, western blot, immunohistochemistry or other assay...
Keywords: Alzheimer Disease, Cancer, Chromatin immunoprecipitation, Diagnostic, Enzyme-linked immunosorbent assay, Flow cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Neuroscience, Western blot

Peer-Reviewed Papers


Related Products

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?