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T1503 Sigma

Trizma® base

Primary Standard and Buffer, ≥99.9% (titration), crystalline

Synonym: 2-Amino-2-(hydroxymethyl)-1,3-propanediol, THAM, Tris base, Tris(hydroxymethyl)aminomethane, Trometamol

  • CAS Number 77-86-1

  • Linear Formula NH2C(CH2OH)3

  • Molecular Weight 121.14

  •  Beilstein Registry Number 741883

  •  EC Number 201-064-4

  •  MDL number MFCD00004679

  •  PubChem Substance ID 24899973

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Properties

Related Categories Biochemicals and Reagents, Biological Buffers, Buffers, Buffers / Buffer salts, Buffers A to Z,
Quality Level   ELITE
description   aminopeptidase substrate
InChI Key   LENZDBCJOHFCAS-UHFFFAOYSA-N
assay   ≥99.9% (titration)
form   crystalline
impurities   ≤0.2% water (Karl Fischer)
pH   10.5-12
useful pH range   7 - 9
pKa (25 °C)   8.1
bp   219-220 °C/10 mmHg(lit.)
mp   167-172 °C(lit.)
solubility   water: soluble (678 g/l at 20 °C)
absorption   A40%/290 nm ≤0.05
Featured Industry   Diagnostic Assay Manufacturing

Description

Other Notes

The pH values of all buffers are temperature- and concentration-dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution.
For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.

Go to Redi-Dri Product Listing RDD008

Packaging

1 kg in poly bottle

25, 100, 250, 500 g in poly bottle

5, 10, 25, 50 kg in poly drum

Application

Trizma base has been used-
• in carotenoid extraction from tubers
• along with Tween, for the saturation of microplate well prior to double sandwich ELISA (enzyme linked immunosorbent assay)
• as a component of sample buffer during protein extraction prior to Western blotting
• as a component of assay buffer during protein extraction prior to radioimmunoassay

General description

40% (w/w) solution in water is clear and colorless.

Legal Information

Redi-Dri is a trademark of Sigma-Aldrich Co. LLC

Trizma is a registered trademark of Sigma-Aldrich Co. LLC

Safety & Documentation

Safety Information

Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
1
RTECS 
TY2900000

Frequently Asked Questions

What is the pH of Trizma® base, T1503?
The pH of a 0.1 M aqueous solution of Trizma base will have a pH of approximately 10.4.
What type of electrode should I use to measure the pH of Trizma® base T1503?
We recommend the use of glass-calomel electrodes for use with Trizma base solutions through a pH range of 0-12 and a temperature range of -5 °C to 80 °C. Silver/silver chloride reference electrodes are not recommended for use with Trizma solutions that contain proteins.
How can I use T1503 to make a Trizma® buffered solution?
Trizma base may be used directly in preparing a buffer by adjusting the pH with a 0.1 to 1.0 M HCl or blended with Trizma hydrochloride.
Can Product T1503, Trizma® base be used as a standard?
Trizma base meets the requirements for a primary basimetric standard.
Can I autoclave solutions that contain Product T1503, Trizma® base?
Yes, Trizma buffered solutions may be autoclaved.
Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
How do I get lot-specific information or a Certificate of Analysis?
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
How do I find price and availability?
There are several ways to find pricing and availability for our products.Once you log onto our website, you will find the price and availability displayed on the product detail page.You can contact any of our Customer Sales and Service offices to receive a quote. USA customers: 1-800-325-3010 orview local office numbers.
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
My question is not addressed here, how can I contact Technical Service for assistance?
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