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T3194 Sigma-Aldrich

Tetanus toxin from Clostridium tetani

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Properties

Related Categories Bacteria, Bioactive Small Molecule Alphabetical Index, Bioactive Small Molecules, Cell Biology, Cell Signaling and Neuroscience,
Quality Level   100
mol wt   150 kDa (heavy chain (100 kDa) and a light chain (50 kDa) connected through an interchain disulfide bond)
solubility   H2O: soluble
storage temp.   2-8°C
Gene Information   Clostridium tetani E88 ... tetX(1061100)

Description

General description

Tetanus toxin is also known as tetanospasmin. It is a 150-kDa protein, produced by Clostridium tetani. This protein consists of a light and a heavy chain.

Application

Tetanus toxin from Clostridium tetani has been used to inhibit vesicular release in Pannexin 1-transfected Neuro2A cells1.

Tetanus toxin from Clostridium tetani has been used as the exocytosis inhibitor/Golgi-dependent secretion to examine whether secretory carrier membrane protein (SCAMP5) stimulated α-synuclein secretion via the conventional Golgi-dependent pathway. It has also been used in peripheral blood mononuclear cells (PBMCs) culture for T cell proliferation screening.

Biochem/physiol Actions

Binds to polysialogangliosides acceptors on surface of all peripheral presynaptic nerve terminals. Internalized by receptor-mediated endocytosis and carried to spinal cord and brain by transsynaptic retrograde transport. Blocks the release of glycine from inhibitory interneurons of the spinal cord. Cleaves synaptobrevin (VAMP-2), and blocks synaptic vesicle exocytosis in vivo.

Tetanus toxin can cause spastic paralysis.

Preparation Note

Purified from cultures of Clostridium tetani by a modification of the method of Robinson, et al.

Reconstitution

Each vial, when reconstituted with 100 μL of sterile purified water, contains 25 μg of tetanus toxin in 20 mM HEPES, pH 7.4 with 1.25% lactose.

Analysis Note

Two bands by SDS-PAGE under reducing conditions (100 kDa heavy chain and 50 kDa light chain).

Safety & Documentation

Safety Information

Symbol 
GHS06  GHS06
Signal word 
Danger
Hazard statements 
RIDADR 
UN3462 - class 6.1 - PG 1 - Toxins, extracted from living sources, solid, n.o.s., HI: all (not BR)
WGK Germany 
3

Documents

Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Protocols & Articles
Peer-Reviewed Papers
15

References

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