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T5941 Sigma-Aldrich

Trizma® hydrochloride

BioPerformance Certified, suitable for cell culture, ≥99.0% (titration)

Synonym: TRIS HCl, TRIS hydrochloride, Tris(hydroxymethyl)aminomethane hydrochloride, Tromethane hydrochloride

  • CAS Number 1185-53-1

  • Linear Formula NH2C(CH2OH)3 · HCl

  • Molecular Weight 157.60

  •  Beilstein/REAXYS Number 3675235

  •  EC Number 214-684-5

  •  MDL number MFCD00012590

  •  PubChem Substance ID 24900332

  •  NACRES NA.25

Purchase

Properties

Related Categories Apoptosis and Cell Cycle, BioPerformance Certified Buffers, Biochemicals and Reagents, Biological Buffers, Buffer / Buffer Salts,
Quality Level   400
grade   BioPerformance Certified
assay   ≥99.0% (titration)
form   crystalline powder
application(s)   cell culture | mammalian: suitable
  electrophoresis: suitable
impurities   DNAse, Exonuclease; NICKase, Endonuclease; RNAse and Protease, none detected
  endotoxin and total aerobic microbial count, tested
  ≤0.5% water (Karl Fischer)
useful pH range   7.0 - 9.0
pKa (25 °C)   8.1
mp   150-152 °C
solubility   H2O: 667 mg/mL
absorption   ≤0.05 at 290 at 40%
suitability   suitable for electrophoresis
Featured Industry   Diagnostic Assay Manufacturing
SMILES string   Cl.NC(CO)(CO)CO
InChI   1S/C4H11NO3.ClH/c5-4(1-6,2-7)3-8;/h6-8H,1-3,5H2;1H
InChI key   QKNYBSVHEMOAJP-UHFFFAOYSA-N

Description

Application

The pH values of all buffers are temperature and concentration dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution.
For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.

Packaging

1 kg in poly bottle

100, 500 g in poly bottle

5 kg in poly drum

Other Notes

Easily compare specifications for Trizma HCl products with the Trizma HCl specification table.

Legal Information

Trizma is a registered trademark of Sigma-Aldrich Co. LLC

Safety & Documentation

Safety Information

Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
1

pHast Pack
Protocols & Articles

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Peer-Reviewed Papers
15

References

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