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T6066 Sigma

Trizma® base

BioPerformance Certified, meets EP, USP testing specifications, cell culture tested, ≥99.9% (titration)

Synonym: 2-Amino-2-(hydroxymethyl)-1,3-propanediol, THAM, Tris base, Tris(hydroxymethyl)aminomethane, Trometamol

  • CAS Number 77-86-1

  • Linear Formula NH2C(CH2OH)3

  • Molecular Weight 121.14

  •  Beilstein Registry Number 741883

  •  EC Number 201-064-4

  •  MDL number MFCD00004679

  •  PubChem Substance ID 24900345

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Properties

Related Categories BioPerformance Certified Buffers, Biochemicals and Reagents, Biological Buffers, Buffer / Buffer Salts, Buffered Salt Solutions,
Quality Level   ELITE
grade   BioPerformance Certified
description   aminopeptidase substrate
InChI Key   LENZDBCJOHFCAS-UHFFFAOYSA-N
assay   ≥99.9% (titration)
form   crystalline powder
impurities   DNase, RNase, protease, none detected
  Endotoxin and Total Count Aerobic, Yeast, plus Mold, tested
  ≤0.2% water (Karl Fischer)
loss   ≤0.5% (loss on drying, 110 °C)
pH   10.0-11.5
useful pH range   7 - 9
pKa (25 °C)   8.1
bp   219-220 °C/10 mmHg(lit.)
mp   167-172 °C(lit.)
solubility   water: soluble (666 mg/ml, yielding a clear colorless solution)
absorption   A1M/260 ≤0.05
  A1M/280 ≤0.06
  A40%/290 ≤0.05
suitability   cell culture tested
  meets EP, USP testing specifications
Featured Industry   Diagnostic Assay Manufacturing

Description

Other Notes

Easily compare specifications for Trizma products with the Trizma specification table.

The pH values of all buffers are temperature- and concentration-dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution.
For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.

Packaging

1 kg in poly bottle

100, 500 g in poly bottle

5 kg in poly drum

Legal Information

Trizma is a registered trademark of Sigma-Aldrich Co. LLC


High Quality GMP-Buffers
Protocols & Articles

Articles

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While several genome editing tools have been developed in recent years, including zinc finger-based strategies and TALENs (transcription activator-like effector nucleases), none have been as efficien...
Keywords: Amplification, Cell culture, Enzyme-linked immunosorbent assay, Gene expression, Genetic, Genetics, Immunohistochemistry, Immunology, Nutrition, Polymerase chain reaction, Recombination, Southern blot, Transcription, Transfection, Western blot

Protocols

A general protocol for the generation of Nanobodies for structural biology

Els Pardon a, b , Toon Laeremans a, b , Sarah Triest a, b , Søren G F Rasmussen c , Alexandre Wohlkönig a, b , Armin Ruf d , Serge Muyldermans b, e , Wim G J Hol f , Brian K Kobilka g , Jan Steyaert ...
Keywords: AGE, Adsorption, Amplification, Antibiotics, Biochemistry, Biotinylations, Buffers, Cancer, Centrifugation, Chromatography, Cloning, Coagulation, Crystallization, DNA purification, Deaminations, Degradations, Detergents, Digestions, Diseases, Drug discovery, Electrophoresis, Enzyme-linked immunosorbent assay, Flow cytometry, Gel electrophoresis, Gene expression, Genetic, Genomics, Hematology, High performance liquid chromatography, Immobilization, Immunology, Infectious Diseases, Neuroscience, Nucleic acid annealing, Nucleic acid hybridization, Peptide synthesis, Phase transitions, Polymerase chain reaction, Purification, Sample preparations, Separation, Sequencing, Size-exclusion chromatography, Sterilizations, Transfection, Western blot, transformation

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Buffer Reference Center

Citric Acid – Na2HPO4 Buffer Solutions, pH approx. 2.6–7.61 Citric acid monohydrate, C6H8O7 • H2O, M. wt. 210.14; 0.1M-solution contains 21.01 g/l. Na2HPO4, M. wt. 141.98; 0.2M-solution contains 28.4...
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缓冲液参考中心

柠檬酸 – Na2HPO4 缓冲液,pH约为 2.6–7.61 柠檬酸一水合物, C6H8O7 • H2O, M. wt. 210.14; 0.1M的溶液中含有21.01 g/l。 Na2HPO4, M. wt. 141.98; 0.2M的溶液中含有28.40g / l 或 Na2HPO4 • 2H2O, M. wt. 178.05; 0.2M的溶液中含有35.61 g/l。 xml 0.1M的...

Peer-Reviewed Papers
15

References

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