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T6066 Sigma-Aldrich

Trizma® base

BioPerformance Certified, meets EP, USP testing specifications, suitable for cell culture, ≥99.9% (titration)

Synonym: 2-Amino-2-(hydroxymethyl)-1,3-propanediol, THAM, Tris base, Tris(hydroxymethyl)aminomethane, Trometamol

  • CAS Number 77-86-1

  • Linear Formula NH2C(CH2OH)3

  • Molecular Weight 121.14

  •  Beilstein/REAXYS Number 741883

  •  EC Number 201-064-4

  •  MDL number MFCD00004679

  •  PubChem Substance ID 24900345

  •  NACRES NA.25

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Properties

Related Categories BioPerformance Certified Buffers, Biochemicals and Reagents, Biological Buffers, Buffer / Buffer Salts, Buffered Salt Solutions,
Quality Level   400
grade   BioPerformance Certified
description   aminopeptidase substrate
assay   ≥99.9% (titration)
form   crystalline powder
application(s)   cell culture | mammalian: suitable
impurities   DNase, RNase, protease, none detected
  Endotoxin and Total Count Aerobic, Yeast, plus Mold, tested
  ≤0.2% water (Karl Fischer)
loss   ≤0.5% (loss on drying, 110 °C)
pH   10.0-11.5
useful pH range   7 - 9
pKa (25 °C)   8.1
bp   219-220 °C/10 mmHg (lit.)
mp   167-172 °C (lit.)
solubility   water: soluble (666 mg/ml, yielding a clear colorless solution)
absorption   ≤0.05 at 260 at 1 M
  ≤0.05 at 290 at 40%
  ≤0.06 at 280 at 1 M
suitability   meets EP, USP testing specifications
Featured Industry   Diagnostic Assay Manufacturing
SMILES string   NC(CO)(CO)CO
InChI   1S/C4H11NO3/c5-4(1-6,2-7)3-8/h6-8H,1-3,5H2
InChI key   LENZDBCJOHFCAS-UHFFFAOYSA-N

Description

General description

Tris is an established basimetric standard and buffer used in biochemistry and molecular biology. It may be used by itself as a buffer or as a component of mixed buffer formulations, such as Tris-EDTA (TE) buffer, Tris-acetate-EDTA (TAE) buffer, Tris-borate-EDTA (TBE) buffer, etc. It is pure, essentially stable, relatively non-hygroscopic and has a high equivalent weight.

Application

Trizma has been used:
• As a component in the culture of human embryonic stem (hES) cells on human and mouse feeder cells
• As a component of pre-hybridization buffer in in situ hybridization
• To suspend dried protein before SDS-PAGE protein characterization
• As a component of lysis buffer to wash and lyse cells before immunoprecipitation
• In the extraction and enrichment of membrane proteins for separation by two-dimensional polyacrylamide gel electrophoresis
• In studies of double stranded complexes of peptide nucleic acids (PNA) and their complementary DNA sequences, by use of anion exchange HPLC
• In capillary electrochromatography and UV analysis of tocopherols and tocotrienols

Packaging

1 kg in poly bottle

100, 500 g in poly bottle

5 kg in poly drum

Other Notes

Easily compare specifications for Trizma products with the Trizma specification table.

The pH values of all buffers are temperature- and concentration-dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution.
For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.

Legal Information

Trizma is a registered trademark of Sigma-Aldrich Co. LLC

Safety & Documentation

Safety Information

Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
1
RTECS 
TY2900000

pHast Pack
Protocols & Articles

Articles

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Peer-Reviewed Papers
15

References

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